{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,26]],"date-time":"2026-07-26T13:00:29Z","timestamp":1785070829560,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32572808"],"award-info":[{"award-number":["32572808"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Glutathione\n                    <jats:italic>S<\/jats:italic>\n                    \u2010transferases (GSTs) constitute a critical enzyme superfamily responsible for xenobiotic detoxification in insects. The green peach aphid,\n                    <jats:italic>Myzus persicae<\/jats:italic>\n                    , is a globally significant agricultural pest that has exhibited declining field susceptibility to \u03bb\u2010cyhalothrin (LCT), a widely applied pyrethroid insecticide. While elevated GST activity has been correlated with LCT tolerance in various aphid populations, the precise molecular mechanisms driving this process have not been fully elucidated. In this study, we functionally characterized a delta\u2010class GST gene (designated\n                    <jats:italic>MpGSTd1<\/jats:italic>\n                    ) from\n                    <jats:italic>M. persicae<\/jats:italic>\n                    . The encoded protein possesses hallmark structural motifs of the GST family, including distinct glutathione\u2010binding (G\u2010site) and hydrophobic substrate\u2010binding (H\u2010site) pockets. Transcriptional profiling revealed maximal\n                    <jats:italic>MpGSTd1<\/jats:italic>\n                    expression during the late nymphal to adult stages, and its transcript levels were markedly induced following LCT challenge. Biochemical analysis of the recombinant MpGSTd1 protein confirmed its catalytic competency toward the model substrate CDNB, exhibiting a\n                    <jats:italic>V<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    of 0.91 \u00b5mol\/min\/mg and a\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    of 2.73\u2009mM. Notably, LCT exposure inhibited the enzyme's activity\n                    <jats:italic>in vitro<\/jats:italic>\n                    with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 0.02\u2009mM, and high\u2010performance liquid chromatography (HPLC) assays demonstrated that MpGSTd1 can directly deplete LCT by 11.4%. Furthermore, RNA interference (RNAi)\u2010mediated suppression of\n                    <jats:italic>MpGSTd1 in vivo<\/jats:italic>\n                    significantly elevated aphid mortality upon subsequent LCT exposure. Collectively, these findings delineate an essential role for\n                    <jats:italic>MpGSTd1<\/jats:italic>\n                    in modulating LCT susceptibility in\n                    <jats:italic>M. persicae<\/jats:italic>\n                    and underscore its potential utility as a molecular target for future aphid management strategies.\n                  <\/jats:p>","DOI":"10.1002\/arch.70184","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:02:30Z","timestamp":1782856950000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Delta\u2010Class Glutathione\n                    <i>S<\/i>\n                    \u2010Transferase Modulates the Susceptibility of\n                    <i>Myzus persicae<\/i>\n                    to \u03bb\u2010Cyhalothrin"],"prefix":"10.1002","volume":"122","author":[{"given":"Wang\u2010Xi","family":"Shen","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Agri\u2010products Quality and Biosafety, School of Plant Protection Anhui Agricultural University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Min","family":"Yu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Agri\u2010products Quality and Biosafety, School of Plant Protection Anhui Agricultural University Hefei Anhui China"},{"name":"Sichuan Branch of China National Tobacco Corporation Sichuan Tobacco Science Institute Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Agri\u2010products Quality and Biosafety, School of Plant Protection Anhui Agricultural University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Xing","family":"Zhang","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Agri\u2010products Quality and Biosafety, School of Plant Protection Anhui Agricultural University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5327-4011","authenticated-orcid":false,"given":"Mao\u2010Ye","family":"Li","sequence":"additional","affiliation":[{"name":"MOE Key Laboratory of Agri\u2010products Quality and Biosafety, School of Plant Protection Anhui Agricultural University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2010Xin","family":"Chen","sequence":"additional","affiliation":[{"name":"Hainan Branch of China National Tobacco Corporation Haikou Cigar Research Institute Haikou Hainan 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