{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:03:51Z","timestamp":1787238231995,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170511"],"award-info":[{"award-number":["32170511"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Spermatogenesis is a conserved process across animals, involving the maintenance and differentiation of germ stem cells, haploid spermatid production via meiosis, and morphologically distinct sperm formation during spermiogenesis. We previously demonstrated that\n                    <jats:italic>Slmap<\/jats:italic>\n                    (Sarcolemma\u2010associated protein) knockdown causes male sterility with no sperm in the seminal vesicle. Here, we show that\n                    <jats:italic>Slmap<\/jats:italic>\n                    knockdown in the early\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    germline led to severe spermiogenesis defects, including aberrant paracrystalline material deposition in both mitochondrial derivatives, fusion of the two derivatives and of spermatids, loss of the central pair of axonemal microtubules, and axoneme clustering. These phenotypes were supported by transcriptomic data showing that the most prominently affected genes were those involved in cuticle development and plasma\u2010membrane adhesion, including 20 cuticular protein genes. In parallel, we detected upregulation of individualization complex genes (\n                    <jats:italic>chic<\/jats:italic>\n                    ,\n                    <jats:italic>ctp<\/jats:italic>\n                    ,\n                    <jats:italic>WASp<\/jats:italic>\n                    ,\n                    <jats:italic>Lasp<\/jats:italic>\n                    ) and significant association with the oxidative phosphorylation pathway. Consistently, KEGG analysis and functional assays showed that the sustained damage activated germ cell apoptosis, as indicated by increased TUNEL signals, elevated\n                    <jats:italic>Hid<\/jats:italic>\n                    levels, and reduced\n                    <jats:italic>Diap1<\/jats:italic>\n                    expression. Together, these findings place Slmap at the intersection of structural integrity and cell survival, linking its loss to both morphogenetic failure and activation of the\n                    <jats:italic>Hid<\/jats:italic>\n                    \u2010\n                    <jats:italic>Diap1<\/jats:italic>\n                    apoptotic pathway.\n                  <\/jats:p>","DOI":"10.1002\/arch.70186","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:01:14Z","timestamp":1782856874000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Slmap<\/i>\n                    Is Required For Spermiogenesis in\n                    <i>Drosophila melanogaster<\/i>"],"prefix":"10.1002","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5332-7002","authenticated-orcid":false,"given":"Bin","family":"Mao","sequence":"first","affiliation":[{"name":"Key Laboratory of Pesticide &amp; Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences Central China Normal University Wuhan China"},{"name":"Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, School of Biology and Agricultural Resources Huanggang Normal University Huanggang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u2010Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide &amp; Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences Central China Normal University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wan\u2010Yi","family":"Dong","sequence":"additional","affiliation":[{"name":"Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, School of Biology and Agricultural Resources Huanggang Normal University Huanggang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Pesticide &amp; Chemical Biology of Ministry of Education, Hubei Key Laboratory of Genetic Regulation and Integrative Biology, School of Life Sciences Central China Normal University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00120-5"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.30278"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.022269"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21779"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-020-0183-x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21209"},{"key":"e_1_2_9_8_1","first-page":"2861","volume-title":"Development","author":"Cuevas 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