{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T08:18:36Z","timestamp":1787818716280,"version":"build-2784847793"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000159","name":"Natural Resources Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000159","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Heat shock proteins (HSPs) are essential across several insect species for adaptation to environmental stressors. Most pest insects experience fertilization concurrently with summer heat extremes, which mostly affect male testis development and spermatogenesis; although the molecular mechanisms involved are unclear. Several HSPs are exclusive to the testis in several insects, but their functional roles remain unclear. In this study, we identified a testis\u2010specific ATP\u2010dependent HSP,\n                    <jats:italic>CfHSP70\u20102<\/jats:italic>\n                    in the spruce budworm (SBW),\n                    <jats:italic>Choristoneura fumiferana<\/jats:italic>\n                    . Reverse transcript\u2010qualitative polymerase chain reaction and western blotting were performed to detect the tissue\u2010and developmental stage\u2010specific expression of CfHSP70\u20102. In addition, the effects of various stressors on CfHSP70\u20102 expression in the testes were examined. Importantly, CfHSP70\u20102 was first detected in the late pupal stages and peaked in the adult stages. Heating, starvation, or chemical HSP activators did not stimulate CfHSP70\u20102 expression. Immunohistochemical staining revealed that CfHSP70\u20102 was primarily found in round spermatids at the late pupal and adult stages. The potential function role of CfHSP70\u20102 in spermatogenesis in SBW is discussed.\n                  <\/jats:p>","DOI":"10.1002\/arch.70192","type":"journal-article","created":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T18:04:57Z","timestamp":1787681097000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression and Localization of a Testis\u2010Specific Heat Shock Protein (CfHSP70\u20102) During Spermatogenesis in the Spruce Budworm,\n                    <i>Choristoneura fumiferana<\/i>\n                    (Clem.)"],"prefix":"10.1002","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1888-7530","authenticated-orcid":false,"given":"Guoxing","family":"Quan","sequence":"first","affiliation":[{"name":"Natural Resources Canada, Canadian Forest Service, Great Lakes Forestry Centre Marie Ontario 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