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While functionally characterized in some leaf beetle species (e.g.,\n                    <jats:italic>Leptinotarsa<\/jats:italic>\n                    ), its molecular mechanisms and physiological roles in many agriculturally important Chrysomelids remain poorly understood. This study provides the first comprehensive functional analysis of\n                    <jats:italic>MhieJHEH<\/jats:italic>\n                    in the maize pest\n                    <jats:italic>Monolepta hieroglyphica<\/jats:italic>\n                    . We cloned and sequenced the gene, which encodes a 454\u2010amino acid protein classified within the Coleoptera JHEH clade. Structural modeling revealed a binding pocket for JH III by key residues Thr250 and Arg379, confirmed by enzymatic assays showing the protein catalyzes JH III conversion. Expression analysis indicated significantly higher transcript levels in the female abdomen, thorax, cuticle, and intestine, implying a sex\u2010biased regulatory function. RNAi\u2010mediated knockdown of\n                    <jats:italic>MhieJHEH<\/jats:italic>\n                    disrupted JH III homeostasis and caused severe adult mortality, establishing its essential role in survival. Integrated transcriptomic and proteomic analyses following knockdown revealed extensive dysregulation of genes and proteins enriched in metabolic pathways, oxidative phosphorylation, and energy derivation, with strong correlations in mitochondrial and nucleoside metabolism components. These findings establish\n                    <jats:italic>MhieJHEH<\/jats:italic>\n                    as a critical regulator of JH degradation, adult survival, and systemic metabolism in\n                    <jats:italic>M. hieroglyphica<\/jats:italic>\n                    , supporting its potential as a target for novel pest management strategies.\n                  <\/jats:p>","DOI":"10.1002\/arch.70198","type":"journal-article","created":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:07:35Z","timestamp":1787508455000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["RNAi Targeting\n                    <i>MhieJHEH<\/i>\n                    Disrupts Hormonal Homeostasis and Induces Systemic Metabolic Dysregulation in\n                    <i>Monolepta hieroglyphica<\/i>"],"prefix":"10.1002","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9912-0843","authenticated-orcid":false,"given":"Xuan","family":"Song","sequence":"first","affiliation":[{"name":"Zhongyuan Research Center Chinese Academy of Agricultural Sciences Xinxiang Henan China"},{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-9748-4028","authenticated-orcid":false,"given":"Yi\u2010meng","family":"Pi","sequence":"additional","affiliation":[{"name":"Zhongyuan Research Center Chinese Academy of Agricultural Sciences Xinxiang Henan China"},{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3398-0382","authenticated-orcid":false,"given":"Sheng\u2010jie","family":"Han","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9162-301X","authenticated-orcid":false,"given":"Xiao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhongyuan Research Center Chinese Academy of Agricultural Sciences Xinxiang Henan China"},{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7085-7224","authenticated-orcid":false,"given":"Jun\u2010feng","family":"Kou","sequence":"additional","affiliation":[{"name":"Hebei Key Laboratory of Soil Entomology CangZhou Academy of Agriculture and Forestry Sciences CangZhou Hebei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4766-4889","authenticated-orcid":false,"given":"Yue\u2010qin","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7746-7931","authenticated-orcid":false,"given":"Tian\u2010tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4276-9072","authenticated-orcid":false,"given":"Yong\u2010jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhongyuan Research Center Chinese Academy of Agricultural Sciences Xinxiang Henan China"},{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,23]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.121.056589"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.123.21364"},{"key":"e_1_2_10_4_1","first-page":"28","article-title":"Fitting a Mixture Model by Expectation Maximization to Discover Motifs in Biopolymers","volume":"2","author":"Bailey T. 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