{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T08:18:33Z","timestamp":1787818713709,"version":"build-2784847793"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.947511\/2024\u201000"],"award-info":[{"award-number":["88887.947511\/2024\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["302871\/2022\u20105"],"award-info":[{"award-number":["302871\/2022\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["309890\/2022\u20105"],"award-info":[{"award-number":["309890\/2022\u20105"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["408598\/2023\u20109"],"award-info":[{"award-number":["408598\/2023\u20109"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ\u201005316\u201023"],"award-info":[{"award-number":["APQ\u201005316\u201023"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The Neotropical stink bugs\n                    <jats:italic>Euschistus heros<\/jats:italic>\n                    and\n                    <jats:italic>Diceraeus melacanthus<\/jats:italic>\n                    are major pests of soybean and maize in South America, yet current chemical control strategies face widespread resistance, highlighting the urgent need for sustainable alternatives. This study investigates\n                    <jats:italic>Ficus carica<\/jats:italic>\n                    pruning residues as a source of selective botanical insecticides, integrating optimized extraction, phytochemical profiling, bioassays, and molecular modeling. Eight extracts were prepared under varying solvent, temperature, and acidity conditions, and analyzed for total phenolic content (TPC) and the key furanocoumarins psoralen and bergapten. Acidification enhanced overall mass yields, while mild ethanol extraction at room temperature selectively maximized furanocoumarin recovery, yielding psoralen concentrations up to 14.83\u2009mg\u2009g\n                    <jats:sup>\u22121<\/jats:sup>\n                    , which is substantially higher than previously reported in leaves or fruit. Biological evaluation of the optimized ethanolic extract (ERA) revealed strong insecticidal activity, with 86% mortality of\n                    <jats:italic>E. heros<\/jats:italic>\n                    and 40% of\n                    <jats:italic>D. melacanthus<\/jats:italic>\n                    nymphs at 48\u2009h, and a calculated LC\n                    <jats:sub>50<\/jats:sub>\n                    of 1232\u2009mg\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    for\n                    <jats:italic>E. heros<\/jats:italic>\n                    . The differential susceptibility between species suggests both metabolic and cuticular factors influence efficacy. Computational docking and phylogenetic analyses suggested a potential mechanistic basis for the observed selectivity: furanocoumarins are predicted to bind hemipteran AChE via a compensatory polar scaffold, whereas binding to\n                    <jats:italic>Apis mellifera<\/jats:italic>\n                    AChE is predicted to be weaker due to lineage\u2010specific differences in aromatic density within the catalytic gorge, potentially explaining the minimal off\u2010target susceptibility. The molecular modeling results characterize these compounds as low\u2010affinity, reversible inhibitors, combining effective pest control with a favorable safety profile for pollinators. The present work demonstrates that valorizing agro\u2010industrial waste from\n                    <jats:italic>F. carica<\/jats:italic>\n                    can yield potent, selective, and environmentally safer insecticidal agents. The integration of extraction optimization, biological evaluation, and molecular modeling provides a robust framework for developing sustainable botanical insecticides, advancing circular economy principles in pest management and offering promising alternatives to synthetic neurotoxins.\n                  <\/jats:p>","DOI":"10.1002\/arch.70200","type":"journal-article","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T11:41:31Z","timestamp":1785584491000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Valorization of\n                    <i>Ficus carica<\/i>\n                    Pruning Residues as Selective Botanical Insecticides: Optimized Furanocoumarin Extraction, Efficacy Against Neotropical Stink Bugs, and Mechanistic Insights via Molecular Docking"],"prefix":"10.1002","volume":"122","author":[{"given":"Thais A.","family":"Almeida","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Vi\u00e7osa, University Campus Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0471-4622","authenticated-orcid":false,"given":"Arley R.","family":"P\u00e1ez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Vi\u00e7osa, University Campus Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lara T. M.","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Entomology Federal University of Vi\u00e7osa, University Campus Vi\u00e7osa Minas Gerais Brazil"},{"name":"Institute of Entomology Guizhou University Guiyang Guizhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8334-3313","authenticated-orcid":false,"given":"Guy","family":"Smagghe","sequence":"additional","affiliation":[{"name":"Institute of Entomology Guizhou University Guiyang Guizhou China"},{"name":"Department of Biology Vrije Universiteit Brussel (VUB) Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yara M.","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Vi\u00e7osa, University Campus Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia M.","family":"Faria","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Vi\u00e7osa, University Campus Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1174-6564","authenticated-orcid":false,"given":"Eug\u00eanio E.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Entomology Federal University of Vi\u00e7osa, University Campus Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3747-4424","authenticated-orcid":false,"given":"Jo\u00e3o Paulo V.","family":"Leite","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Federal University of Vi\u00e7osa, University Campus Vi\u00e7osa Minas Gerais Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,1]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.37231\/myjas.2025.10.1.427"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.31024\/apj.2022.7.1.1"},{"key":"e_1_2_10_5_1","first-page":"41","article-title":"Potential of Leaves of Eighteen Cultivars of Ficus carica as Antioxidants and Profiling of Phenolic Compounds as Active Molecules","volume":"15","author":"Akhtar P.","year":"2019","journal-title":"Iranian Journal of Pharmaceutical Sciences"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsh3.12034"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA16746E"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00320991"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6504"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5167"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm300871x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp163"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules15107313"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005659"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34630-w"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007996124545"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.18596\/jotcsa.1419322"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2017.03.087"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2015.1094804"},{"issue":"6","key":"e_1_2_10_21_1","article-title":"Culex pipiens pallens Cuticular Protein CPLCG5 Participates in Pyrethroid Resistance by Forming a Rigid Matrix","volume":"11","author":"Huang Y.","year":"2018","journal-title":"Parasites & Vectors"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(97)00009-X"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0897"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1107\/S2052252514009324"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.3280"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.33745\/ijzi.2022.v08i01.041"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26052182"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.7745"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-8-306"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1075"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01488-1"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2024.106625"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12298-018-0550-3"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-2946-3-33"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2009.09.004"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10068-013-0185-7"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac993"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.17660\/eJHS.2018\/83.2.5"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781315373775"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6032"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.12688\/f1000research.17456.1"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2013.04.081"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-015-0666-y"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.4103\/0975-7406.148775"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab314"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2020.105319"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/toac013"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00128336"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00894-012-1667-x"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects12070599"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10822-011-9462-9"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btaa018"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.10465"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky427"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886-005-9014-7"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1998.2401"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241713177"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(73)90196-2"}],"container-title":["Archives of Insect Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.70200","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/arch.70200","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.70200","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T07:44:54Z","timestamp":1787816694000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/arch.70200"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":58,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/arch.70200"],"URL":"https:\/\/doi.org\/10.1002\/arch.70200","archive":["Portico"],"relation":{},"ISSN":["0739-4462","1520-6327"],"issn-type":[{"value":"0739-4462","type":"print"},{"value":"1520-6327","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-05-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70200"}}