{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T08:18:34Z","timestamp":1787818714190,"version":"build-2784847793"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Diapause in the Sunn pest,\n                    <jats:italic>Eurygaster integriceps<\/jats:italic>\n                    , is a key seasonal adaptation associated with migration, overwintering survival, and reproductive timing. However, the molecular components associated with this physiological state remain poorly characterized in this hemipteran pest. In this study, we identified and characterized a takeout\u2010family transcript from\n                    <jats:italic>E. integriceps<\/jats:italic>\n                    (EiTO1; GenBank OP913374.1) and combined evolutionary, structural, and expression analyses to examine its association with seasonal physiological states. EiTO1 contained a 738\u2010bp open reading frame encoding a 245\u2010amino acid protein and was assigned to the juvenile hormone\u2010binding protein (JHBP) superfamily based on conserved\u2010domain analysis. Phylogenetic reconstruction recovered EiTO1 as sister to the\n                    <jats:italic>Nilaparvata lugens<\/jats:italic>\n                    \u2013\n                    <jats:italic>Bemisia tabaci<\/jats:italic>\n                    clade; however, the low bootstrap support for this placement prevented confident inference of its closest evolutionary relationship. Structural comparison of the predicted EiTO1 model with experimentally resolved takeout\/JHBP\u2010family proteins indicated a conserved barrel\u2010like fold and a corresponding internal cavity, consistent with structural features of this protein family. Quantitative PCR profiling revealed marked seasonal and tissue\u2010specific variation in EiTO1 expression. In female whole\u2010body samples, EiTO1 transcript abundance was low during spring field activity and in newly emerged adults sampled in June, reached its maximum during the refuge\u2010associated phase in August (160\u2010fold), and showed a secondary increase during the late\u2010overwintering\/premigration transition in March (21.5\u2010fold). Tissue\u2010resolved assays in females showed the highest expression in the head and intermediate expression in the fat body. Ovarian expression remained at or below the April baseline at most sampling points but showed a transient increase in August. Together, these findings identify EiTO1 as a takeout\/JHBP\u2010family gene whose expression is associated with seasonal physiological transitions in\n                    <jats:italic>E. integriceps<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1002\/arch.70203","type":"journal-article","created":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T12:01:11Z","timestamp":1785585671000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization and Seasonal Expression Profiling of a Takeout\/Juvenile Hormone\u2010Binding Protein\u2010Family Gene Associated With Diapause in\n                    <i>Eurygaster integriceps<\/i>"],"prefix":"10.1002","volume":"122","author":[{"given":"Behnaz","family":"Farhadi","sequence":"first","affiliation":[{"name":"Department of Plant Protection, College of Agriculture and Natural Resources University of Tehran Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2709-0061","authenticated-orcid":false,"given":"Azam","family":"Amiri","sequence":"additional","affiliation":[{"name":"Department of Landscape Engineering, Faculty of Geography and Environmental Planning University of Sistan and Baluchestan Zahedan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houshang","family":"Alizadeh","sequence":"additional","affiliation":[{"name":"Department of Agronomy and Plant Breeding, College of Agriculture and Natural Resources University of Tehran Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1766-9355","authenticated-orcid":false,"given":"Ali R.","family":"Bandani","sequence":"additional","affiliation":[{"name":"Department of Plant Protection, College of Agriculture and Natural Resources University of Tehran Karaj Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2023.147911"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-27154-y"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2026.104957"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2009.12.010"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-112408-085356"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232012322"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0109825"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.245329"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.70032"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3920\/QAS2015.0622"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2019.103293"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2023.104569"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20221431"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-35524-7"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.8474"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2022.103721"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2024.101198"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.71508"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.825057"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008288"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00030.2024"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2005.09.008"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2023.105388"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects15080573"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/icb\/icae027"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.3125"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2024.106058"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.962604"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-021-07557-7"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80876-4"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.867621"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky427"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbd.2024.101260"}],"container-title":["Archives of Insect Biochemistry and Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.70203","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/arch.70203","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/arch.70203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T07:44:49Z","timestamp":1787816689000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/arch.70203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8]]},"references-count":33,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1002\/arch.70203"],"URL":"https:\/\/doi.org\/10.1002\/arch.70203","archive":["Portico"],"relation":{},"ISSN":["0739-4462","1520-6327"],"issn-type":[{"value":"0739-4462","type":"print"},{"value":"1520-6327","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8]]},"assertion":[{"value":"2026-06-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70203"}}