{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:44:17Z","timestamp":1791600257990,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32360862"],"award-info":[{"award-number":["32360862"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arch Insect Biochem Physiol"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Wohlfahrtia magnifica<\/jats:italic>\n                    , a worldwide pest, causes myiasis in many domestic animals. Its pheromones and volatiles from Bactrian camels were reported to attract or repel\n                    <jats:italic>W. magnifica<\/jats:italic>\n                    , but the molecular mechanisms are still unclear. A small protein in the insect olfactory system, called odorant binding protein (OBP), plays a crucial role in odor reception. In this study, two\n                    <jats:italic>W. magnifica<\/jats:italic>\n                    odorant\u2010binding proteins (WmagOBPs) genes, WmagOBP42531 and WmagOBP39280, were cloned from the antennae of\n                    <jats:italic>W. magnifica<\/jats:italic>\n                    , and then expressed and purified by prokaryotic expression. Fluorescence binding assays demonstrated that WmagOBP39280 exhibits high binding affinity to methylheptenone, butylbutyrate,\n                    <jats:italic>p\u2010<\/jats:italic>\n                    ethylacetophenone, acetophenone (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>i<\/jats:italic>\n                    <\/jats:sub>\n                    : 4.69, 4.15, 3.48, 4.06), while WmagOBP42531 exhibits high binding affinity to\n                    <jats:italic>p\u2010<\/jats:italic>\n                    ethylacetophenone and dipentene (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>i<\/jats:italic>\n                    <\/jats:sub>\n                    : 4.85, 4.98) and moderate binding to methylheptenone, 1\u2010octene\u20103\u2010ol, acetophenone, ethylbenzene.\n                    <jats:italic>o<\/jats:italic>\n                    \u2010xylene (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>i<\/jats:italic>\n                    <\/jats:sub>\n                    : 7.00, 5.96, 5.72, 7.86, 6.15). Molecular docking and molecular dynamics simulation results suggest that a stable complex could be formed between WmagOBP42531 and 1\u2010octene\u20103\u2010ol, acetophenone,\n                    <jats:italic>p<\/jats:italic>\n                    \u2010ethylacetophenone, or\n                    <jats:italic>o<\/jats:italic>\n                    \u2010xylene; between WmagOBP39280 and acetophenone, butylbutyrate,\n                    <jats:italic>p<\/jats:italic>\n                    \u2010ethylacetophenone, or methylheptenone, with Gibbs free energy (\u0394G) ranging from \u221226.84 to \u221214.7\u2009kcal\/mol. RMSD, RMSF, and radius of gyration suggested the spontaneous binding of WmagOBP42531 and WmagOBP39280 to odor ligands.\n                  <\/jats:p>","DOI":"10.1002\/arch.70205","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T18:15:43Z","timestamp":1786644943000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Recognition of Odorant\u2010Binding Proteins WmagOBP39280 and WmagOBP42531 to\n                    <i>Wohlfahrtia magnifica<\/i>\n                    Pheromones and Bactrian Camel Volatiles"],"prefix":"10.1002","volume":"122","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7775-1254","authenticated-orcid":false,"given":"Yuting","family":"Bai","sequence":"first","affiliation":[{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leifen","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuri","family":"Han","sequence":"additional","affiliation":[{"name":"Quality Safety Center for Agricultural and Animal Products of Inner Mongolia Autonomous Region Hohhot People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huar","family":"Bao","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baoxiang","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haobo","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaning","family":"Li","sequence":"additional","affiliation":[{"name":"Quality Safety Center for Agricultural and Animal Products of Inner Mongolia Autonomous Region Hohhot People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5705-665X","authenticated-orcid":false,"given":"Demtu","family":"Er","sequence":"additional","affiliation":[{"name":"Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease, Ministry of Agriculture and Rural Affairs, College of Veterinary Medicine Inner Mongolia Agricultural University Hohhot People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/finsc.2023.1274197"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-93382-7"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2435.2012.02006.x"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2018.01762"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects15060447"},{"key":"e_1_2_11_7_1","volume-title":"Advances in Insect Physiology","author":"D\u00fcRR V.","year":"2022"},{"key":"e_1_2_11_8_1","first-page":"457","article-title":"Insect Antennal Morphology: The Evolution of Diverse Solutions to Odorant Perception","volume":"91","author":"Elgar M. 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