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The occurrence of arachidonic acid and other C<jats:sub>20<\/jats:sub> polyunsaturated fatty acids in tick salivary glands was confirmed by gas chromatography\u2010mass spectrometry. Arachidonate is located entirely in the phospholipid fraction and is associated exclusively with phosphatidylcholine (PC) and phosphatidylethanolamine (PE). Salivary glands stored and frozen for several months had a similar lipid composition as freshly dissected salivary glands, with the exception of a small amount of free arachidonic acid and an increase in lysophosphatidylcholine. Incubation of salivary gland homogenates with snake venom phospholipase A<jats:sub>2<\/jats:sub> showed that most saturated fatty acids are esterified in the sn\u20101 position of PC and PE, with the unsaturated fatty acids in the sn\u20102 position. Approximately 75% of arachidonic acid is in the sn\u20102 position of PC and PE, adding support to the hypothesis that arachidonic acid is released into the cytoplasm after activation of a phospholipase A<jats:sub>2<\/jats:sub> for subsequent metabolism to prostaglandins and\/or other eicosanoids. \u00a9 1993 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/arch.940230106","type":"journal-article","created":{"date-parts":[[2005,2,24]],"date-time":"2005-02-24T07:53:40Z","timestamp":1109231620000},"page":"37-52","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Analysis of lipids in the salivary glands of <i>Amblyomma americanum<\/i> (L.): Detection of a high level of arachidonic acid"],"prefix":"10.1002","volume":"23","author":[{"given":"Michael M.","family":"Shipley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack W.","family":"Dillwith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Sauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard C.","family":"Essenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph W.","family":"Howard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,2,7]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"22","volume-title":"Morphology, Physiology, and Behavioral Biology of Ticks","author":"Fawcett D. 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