{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T06:07:46Z","timestamp":1764828466355},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,12,27]],"date-time":"2013-12-27T00:00:00Z","timestamp":1388102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:sec><jats:label \/><jats:p>Racemic misonidazole, a radiosensitizer formally used in radiation therapy of cancer and to date still applied, was once reported to exhibit strong inhibitory effects on mouse glutathione peroxidases (GPX). This appeared to qualify misonidazole as a lead structure for the development of novel GPX inhibitors to cause oxidative stress in chemotherapy\u2010resistant tumors. A unique feature of misonidazole as an inhibitor of GPX is the absence of a thiol functionality. Therefore, it was expected to selectively target inhibition devoid of promiscuous interactions with cations and sulfhydryl groups. We synthesized the isomers of misonidazole and analyzed the ability of chiroptical high\u2010performance liquid chromatography (HPLC) to identify the particular enantiomers. Due to the chiral pool synthesis, the assignment of the correct configuration could be verified. Finally, we evaluated both isomers for their inhibitory activities on bovine erythrocyte GPx\u20101, which is 87% homologous to the human enzyme. Despite the previously reported inhibition of racemic misonidazole on the less homologous mouse GPx\u20101, we did not find any significant inhibitory activity on the bovine enzyme for either isomer. Though misonidazole appears unlikely to be an inhibitor of human GPx\u20101 activity, we still spotlight misonidazole as a promising fragment\u2010like lead structure in general.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ardp.201300285","type":"journal-article","created":{"date-parts":[[2013,12,27]],"date-time":"2013-12-27T08:36:47Z","timestamp":1388133407000},"page":"153-160","source":"Crossref","is-referenced-by-count":9,"title":["Evaluation of (<scp><i>S<\/i><\/scp>)\u2010 and (<scp><i>R<\/i><\/scp>)\u2010<scp>M<\/scp>isonidazole as <scp>GPX<\/scp> Inhibitors: Synthesis, Characterization Including Circular Dichroism and <i>In Vitro<\/i> Testing on Bovine <scp>GP<\/scp>x\u20101"],"prefix":"10.1002","volume":"347","author":[{"given":"Felix","family":"Wilde","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy Ernst\u2010Moritz\u2010Arndt University Greifswald Greifswald Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chamseddin","family":"Chamseddin","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy Ernst\u2010Moritz\u2010Arndt University Greifswald Greifswald Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidi","family":"Lemmerhirt","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy Ernst\u2010Moritz\u2010Arndt University Greifswald Greifswald Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick J.","family":"Bednarski","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy Ernst\u2010Moritz\u2010Arndt University Greifswald Greifswald Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Jira","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy Ernst\u2010Moritz\u2010Arndt University Greifswald Greifswald Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Link","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy Ernst\u2010Moritz\u2010Arndt University Greifswald Greifswald Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,12,27]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.1-4-227"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1067\/mcn.2001.120122"},{"key":"e_1_2_5_4_1","first-page":"194","volume":"30","author":"Martin G. 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