{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:54:01Z","timestamp":1788396841570,"version":"build-2803163510"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T00:00:00Z","timestamp":1405382400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:sec><jats:label\/><jats:p>Snake venom 5\u2032\u2010nucleotidase (5\u2032NUC) plays a very important role in envenomation strategies; however, apart from its modulation of hemostatic functions, its other pharmacological effects are not yet well characterized. Several studies have used specific inhibitors of enzyme toxins as a biochemical or pharmacological tool to characterize or establish its mechanism of action. We report here for the first time vanillin mandelic acid (VMA), an analog of vanillin, to potentially, selectively, and specifically inhibit venom 5\u2032NUC activity among other enzymes present in venoms. VMA is much more potent in inhibiting 5\u2032NUC activity than vanillyl acid (VA). The experimental results obtained are in good agreement with the<jats:italic>in silico<\/jats:italic>molecular docking interaction data. Both VA and VMA are competitive inhibitors as evident by the inhibition\u2010relieving effect upon increasing the substrate concentration. VMA also dose\u2010dependently inhibited the anticoagulant effect in<jats:italic>Naja naja<\/jats:italic>venom. In this study, we report novel non\u2010nucleoside specific inhibitors of snake venom 5\u2032NUC and experimentally demonstrate their involvement in the anticoagulant activity of<jats:italic>N. naja<\/jats:italic>venom. Hence, we hypothesize that VMA can be used as a molecular tool to evaluate the role of 5\u2032NUC in snake envenomation and to develop prototypes and lead compounds with potential therapeutic applications against snake bites.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ardp.201400069","type":"journal-article","created":{"date-parts":[[2014,7,15]],"date-time":"2014-07-15T18:43:32Z","timestamp":1405449812000},"page":"616-623","source":"Crossref","is-referenced-by-count":5,"title":["Vanillin Analog \u2013 Vanillyl Mandelic Acid, a Novel Specific Inhibitor of Snake Venom 5\u2032\u2010Nucleotidase"],"prefix":"10.1002","volume":"347","author":[{"given":"Raghaven","family":"Arun","sequence":"first","affiliation":[{"name":"Department of Bioinformatics, SCBT SASTRA University Thirumalaisamudram Thanjavur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul Salam Syed Yasir","family":"Arafat","sequence":"additional","affiliation":[{"name":"Department of Bioinformatics, SCBT SASTRA University Thirumalaisamudram Thanjavur India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cletus J. 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