{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:27:07Z","timestamp":1761514027288,"version":"3.41.2"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T00:00:00Z","timestamp":1498003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007397","name":"Univerzita Karlova v Praze","doi-asserted-by":"publisher","award":["SVV260416","UNCE 204026\/2012"],"award-info":[{"award-number":["SVV260416","UNCE 204026\/2012"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:sec><jats:label\/><jats:p>A series of benzaldehyde and salicylaldehyde\u2010<jats:italic>S<\/jats:italic>\u2010benzylisothiosemicarbazones was synthesized and tested against 12 different strains of mycobacteria, Gram\u2010positive and Gram\u2010negative bacteria, and the significant selectivity toward mycobacteria was proved. Twenty\u2010eight derivatives were evaluated for the inhibition of isocitrate lyase, which is a key enzyme of the glyoxylate cycle necessary for latent tuberculosis infection, and their iron\u2010chelating properties were investigated. Two derivatives, 5\u2010bromosalicylaldehyde\u2010<jats:italic>S<\/jats:italic>\u2010(4\u2010fluorobenzyl)\u2010isothiosemicarbazone and salicylaldehyde\u2010<jats:italic>S<\/jats:italic>\u2010(4\u2010bromobenzyl)\u2010isothiosemicarbazone, influenced the isocitrate lyase activity and caused a better inhibition at 10\u2009\u03bcmol\/L than 3\u2010nitropropionic acid, a standard inhibitor. The compounds were also found to act as exogenous chelators of iron, which is an obligate cofactor for many mycobacterial enzymes. Due to their low cytotoxicity, together with the activity against isocitrate lyase and the ability to sequester iron ions, the compounds belong to potential antibiotics with the main effect on mycobacteria.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ardp.201700020","type":"journal-article","created":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T07:10:41Z","timestamp":1498029041000},"source":"Crossref","is-referenced-by-count":8,"title":["Design, Synthesis, and Biological Evaluation of Isothiosemicarbazones with Antimycobacterial Activity"],"prefix":"10.1002","volume":"350","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9365-3163","authenticated-orcid":false,"given":"Eva","family":"Novotn\u00e1","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy in Hradec Kr\u00e1lov\u00e9, Department of Biochemical Sciences Charles University Hradec Kr\u00e1lov\u00e9 Czech 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