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The chalcones <jats:bold>5a\u2013g<\/jats:bold> were obtained by Claisen\u2013Schmidt condensation of thiazole\u20102\u2010nitrogen mustard <jats:bold>3<\/jats:bold> and selected acetophenones <jats:bold>4a\u2013g<\/jats:bold>. These new compounds <jats:bold>6\/7\/8\/9a\u2013g<\/jats:bold> were screened for their antifungal activity against <jats:italic>Cryptococcus neoformans<\/jats:italic>, with IC<jats:sub>50<\/jats:sub> values of 3.9\u20137.8\u2009\u00b5g\/ml for the <jats:italic>N<\/jats:italic>\u20103,5\u2010dichlorophenyl pyrazolines <jats:bold>9e<\/jats:bold>\u2013<jats:bold>g<\/jats:bold>. Interestingly, those compounds show low cytotoxic effects toward erythrocytes (RBC). In addition, <jats:italic>N<\/jats:italic>\u2010acetyl (<jats:bold>6a,b<\/jats:bold>) and <jats:italic>N<\/jats:italic>\u2010formyl pyrazolines (<jats:bold>7a<\/jats:bold>, <jats:bold>7b<\/jats:bold>, <jats:bold>7c<\/jats:bold>, and <jats:bold>7g<\/jats:bold>) showed inhibitory activity against methicillin\u2010susceptible <jats:italic>Staphylococcus aureus<\/jats:italic>, methicillin\u2010resistant <jats:italic>S. aureus<\/jats:italic>, and vancomycin\u2010intermediate <jats:italic>S. aureus<\/jats:italic>, with the most important minimum inhibitory concentration values ranging from 31.25 to 125\u2009\u00b5g\/ml. Regarding the antiprotozoal activity, thiazolyl\u2010pyrazolines <jats:bold>9g<\/jats:bold>, <jats:bold>8f<\/jats:bold>, and <jats:bold>7c<\/jats:bold> display high activity against <jats:italic>Plasmodium falciparum, Leishmania (V) panamensis<\/jats:italic>, and <jats:italic>Trypanosoma cruzi<\/jats:italic>, with EC<jats:sub>50<\/jats:sub> values of 11.80, 6.46, and 4.98\u2009\u03bcM, respectively, and with <jats:bold>7c<\/jats:bold> being approximately 2.6\u2010fold more potent than benznidazole with a selectivity index of 1.61 on U\u2010937 human cells, showing promising potential as a novel antitrypanosomal agent.<\/jats:p>","DOI":"10.1002\/ardp.201900351","type":"journal-article","created":{"date-parts":[[2020,3,18]],"date-time":"2020-03-18T21:34:13Z","timestamp":1584567253000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["New thiazolyl\u2010pyrazoline derivatives bearing nitrogen mustard as potential 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