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In addition, the inhibitory effects of the newly synthesized compounds on the activities of hCA and acetylcholinesterase (AChE) were investigated in vitro, using the esterase and acetylcholine iodide method. The IC<jats:sub>50<\/jats:sub> values were determined and the <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> values of AChE and hCA activities were calculated from the Lineweaver\u2013Burk graphs determined in this study. The hCA I isoform was inhibited by these chalcone derivatives containing Schiff bases (<jats:bold>3a\u2013j<\/jats:bold> and <jats:bold>5a\u2013f<\/jats:bold>) in low nanomolar levels, whose <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> values ranged between 141.88\u2009\u00b1\u200924.10 and 2,234.47\u2009\u00b1\u200938.11\u2009nM. Against the physiologically dominant isoform hCA II, the compounds demonstrated <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> values varying from 199.31\u2009\u00b1\u200940.45 to 602.79\u2009\u00b1\u2009263.22\u2009nM. Also, these compounds effectively inhibited AChE, with <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> values ranging from 20.41\u2009\u00b1\u20096.04 to 125.94\u2009\u00b1\u200923.88\u2009nM. According to these results, the newly synthesized molecules were found to be potent inhibitors of these enzymes.<\/jats:p>","DOI":"10.1002\/ardp.202000202","type":"journal-article","created":{"date-parts":[[2020,8,20]],"date-time":"2020-08-20T14:13:57Z","timestamp":1597932837000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Synthesis, characterization, and biological studies of chalcone derivatives containing Schiff bases: Synthetic derivatives for the treatment of epilepsy and Alzheimer's disease"],"prefix":"10.1002","volume":"353","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8710-2912","authenticated-orcid":false,"given":"\u00dcmit M.","family":"Ko\u00e7yi\u011fit","sequence":"first","affiliation":[{"name":"Department of Basic Pharmaceutical Sciences, Division of Biochemistry, Faculty of Pharmacy Sivas Cumhuriyet University  Sivas Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3602-7400","authenticated-orcid":false,"given":"Hayreddin","family":"Gezegen","sequence":"additional","affiliation":[{"name":"Department of Nutrition and Dietetics, Faculty of Health Sciences Sivas Cumhuriyet University  Sivas Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3171-0633","authenticated-orcid":false,"given":"Parham","family":"Taslimi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science Bartin University  Bartin Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,8,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.4.1449-1451.2003"},{"key":"e_1_2_7_3_1","first-page":"4","volume":"28","author":"Kocyigit U. 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