{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:37:39Z","timestamp":1761514659623,"version":"3.41.2"},"reference-count":49,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T00:00:00Z","timestamp":1601856000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Entamoeba histolytica<\/jats:italic> is a cosmopolitan protozoan parasite that can produce infections in the intestine and some organs (liver, lungs, and brain), with worldwide prevalence. There are treatments against <jats:italic>E. histolytica<\/jats:italic> (antiparasitics), but as the drugs used in these treatments have presented some type of resistance and\/or side effects, there are cases with complications of this disease. Therefore, it is necessary to develop new drugs aimed at a specific therapeutic target against this parasite. Here, we used the compound 5,5\u2032\u2010[(4\u2010nitrophenyl)methylene]bis(6\u2010hydroxy\u20102\u2010mercapto\u20103\u2010methyl\u20104(3<jats:italic>H<\/jats:italic>)\u2010pyrimidinone) in the patenting process (called D4). D4 has a reported specific use against a glycolytic enzyme, the triosephosphate isomerase of <jats:italic>Trichomonas vaginalis<\/jats:italic> (TvTIM). We determined that D4 has an amoebicidal effect in in vitro cultures, with an IC<jats:sub>50<\/jats:sub> value of 18.5\u2009\u00b5M, and we proposed a specific site of interaction (Lys77, His110, Gln115, and Glu118) in the triosephosphate isomerase of <jats:italic>E. histolytica<\/jats:italic> (EhTIM). Furthermore, compound D4 has favorable experimental and theoretical toxicity results. Therefore, D4 should be further investigated as a potential drug against <jats:italic>E. histolytica<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/ardp.202000263","type":"journal-article","created":{"date-parts":[[2020,10,5]],"date-time":"2020-10-05T14:53:12Z","timestamp":1601909592000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Amoebicidal effect of 5,5\u2032\u2010[(4\u2010nitrophenyl)methylene]bis\u20106\u2010hydroxy\u20102\u2010mercapto\u20103\u2010methyl\u20104(3<i>H<\/i>)\u2010pyrimidinone), a new drug against <i>Entamoeba histolytica<\/i>"],"prefix":"10.1002","volume":"354","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8169-3715","authenticated-orcid":false,"given":"Jos\u00e9 L.","family":"Vique\u2010S\u00e1nchez","sequence":"first","affiliation":[{"name":"Facultad de Medicina Mexicali Universidad Aut\u00f3noma de Baja California Mexicali, Baja California M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albertana","family":"Jim\u00e9nez\u2010Pineda","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n Bioqu\u00edmica ENMyH\u2010Instituto Polit\u00e9cnico Nacional Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia G.","family":"Ben\u00edtez\u2010Cardoza","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n Bioqu\u00edmica ENMyH\u2010Instituto Polit\u00e9cnico Nacional Ciudad de M\u00e9xico M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,5]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"3251","volume":"10","author":"Garc\u00eda Palomo J. 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