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In this study, a triple\u2010targeting inhibitory activity of Rose Bengal against SNTs such as <jats:sc>d<\/jats:sc>\u2010<jats:italic>glycero<\/jats:italic>\u2010\u03b1\u2010<jats:sc>d<\/jats:sc>\u2010<jats:italic>manno<\/jats:italic>\u2010heptose\u20101\u2010phosphate guanylyltransferase (HddC), <jats:sc>d<\/jats:sc>\u2010<jats:italic>glycero<\/jats:italic>\u2010\u03b2\u2010<jats:sc>d<\/jats:sc>\u2010<jats:italic>manno<\/jats:italic>\u2010heptose\u20101\u2010phosphate adenylyltransferase (HldC), and 3\u2010deoxy\u2010<jats:sc>d<\/jats:sc>\u2010<jats:italic>manno<\/jats:italic>\u2010oct\u20102\u2010ulosonic acid cytidylyltransferase (KdsB) from <jats:italic>Burkholderia pseudomallei<\/jats:italic> is provided. Rose Bengal effectively suppresses the nucleotidyltransferase activity of the three SNTs, and its IC<jats:sub>50<\/jats:sub> values are 10.42, 0.76, and 5.31\u2009\u00b5M, respectively. Interestingly, Rose Bengal inhibits the three enzymes regardless of their primary, secondary, tertiary, and quaternary structural differences. The experimental results indicate that Rose Bengal possesses the plasticity to shape its conformation suitable to interact with the three SNTs. As HddC functions in the formation of capsular polysaccharides and HldC and KdsB produce building blocks to constitute the inner core of lipopolysaccharide, Rose Bengal is a potential candidate to design antibiotics in a new category. In particular, it can be developed as a specific antimelioidosis agent. As the mortality rate of the infected people caused by <jats:italic>B. pseudomallei<\/jats:italic> is quite high, there is an urgent need for specific antimelioidosis agents. Therefore, a further study is being carried out with derivatives of Rose Bengal.<\/jats:p>","DOI":"10.1002\/ardp.202000360","type":"journal-article","created":{"date-parts":[[2021,2,9]],"date-time":"2021-02-09T11:49:51Z","timestamp":1612871391000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A triple\u2010targeting inhibitory activity of Rose Bengal on polysaccharide biosynthesis of <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1002","volume":"354","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6461-0981","authenticated-orcid":false,"given":"Suwon","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Pharmacy, College of Pharmacy and Graduate School of Pharmaceutical Sciences Ewha Womans University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seri","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, College of Pharmacy and Graduate School of Pharmaceutical Sciences Ewha Womans University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4092-4203","authenticated-orcid":false,"given":"Mi\u2010Sun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, College of Pharmacy and Graduate School of Pharmaceutical Sciences Ewha Womans University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2205-1453","authenticated-orcid":false,"given":"Dong H.","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, College of Pharmacy and Graduate School of Pharmaceutical Sciences Ewha Womans University Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,2,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2008.03.018"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1998.2961"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2019.0144"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.25398"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2011.05.022"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm301499r"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/329162a0"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja046070d"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2006.02.010"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.620989"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-018-0141-x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2013.01.008"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2013.743991"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/jm500131s"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.69.4.585-607.2005"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01617-06"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8148-1"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/14756366.2020.1751150"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/1087057111421373"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471220639"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2015.8"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(08)70002-6"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1906.120717"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2102.141045"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.107"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.7.3220-3229.2001"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.6b01911"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20133196"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23123152"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1039\/b211266c"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-199607000-00008"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.9247"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0005571"},{"key":"e_1_2_8_35_1","unstructured":"H. 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