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After these isozymes were separately purified, their activities were determined using the esterase activity. IC<jats:sub>50<\/jats:sub> values for hCA I and II were calculated as 2.02\u201311.38\u2009\u00b5M and 2.23\u20139.05\u2009\u00b5M, respectively. The compounds were identified as CA inhibitors, with <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> values in the ranges of 1.66\u20139.17\u2009\u00b5M for the hCA I isozyme and 1.49\u201314.21\u2009\u00b5M for hCA II. The inhibitory effects of these natural compounds were also compared to acetazolamide, which is a potent inhibitor of both CA isozymes. 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