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Results showed that the methanol extract had significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) the highest total phenolic content (TPC; 107.80\u2009mg\u00a0GAE\/g) and total flavonoids content (TFC; 25.21\u2009mg RE\/g) followed by the aqueous extract (102.72\u2009mg GAE\/g and 20.88\u2009mg RE\/g, respectively). LC\u2010MS\/MS\u2010guided profiling of the three extracts revealed that rosmarinic acid (34.8%), hesperetin (42.9%), and linoleic acid (18%) were the dominant compounds in the methanol, aqueous and hexane extracts, respectively. GC\u2010MS analysis of the hexane extract showed that \u0263\u2010sitosterol (29.9%) was the major constituent. The methanol extract displayed significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) the highest Cu<jats:sup>++<\/jats:sup>, Fe<jats:sup>+++<\/jats:sup>, and Mo(VI) ions scavenging and reducing properties while the aqueous extract exerted significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) the highest metal chelating power (42.51\u2009mg EDTAE\/g). Both the hexane and methanol extracts effectively inhibited the acetylcholinesterase enzyme (2.63 and 2.65\u2009mg GALAE\/g, respectively) while the former extract exerted significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05) the highest butyrylcholinesterase (2.32\u2009mg GALAE\/g), tyrosinase (19.73\u2009mg KAE\/g), and amylase (1.16\u2009mmol ACAE\/g) inhibition capacity. The aqueous extract exhibited the best glucosidase inhibition property (0.49\u2009mmol ACAE\/g). The methanol and hexane extracts exerted a higher cytotoxic effect on HT\u201029 (IC<jats:sub>50<\/jats:sub>: 8.12\u2009\u00b5g\/mL) and HeLa (IC<jats:sub>50<\/jats:sub>\u2009=\u20098.08\u2009\u00b5g\/mL) cells, respectively. In conclusion, these results provide valuable insight into the potential use of <jats:italic>T. leucostomus<\/jats:italic> bioactive extracts in different pharmaceutical applications.<\/jats:p>","DOI":"10.1002\/ardp.202300444","type":"journal-article","created":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T12:39:52Z","timestamp":1695818392000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["LC\u2010MS\/MS and GC\u2010MS profiling, antioxidant, enzyme inhibition, and antiproliferative activities of <i>Thymus leucostomus<\/i> H<scp>ausskn<\/scp>. &amp; V<scp>elen<\/scp>. extracts"],"prefix":"10.1002","volume":"356","author":[{"given":"Shaimaa","family":"Fayez","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Ain Shams University  Cairo Abbassia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nouran M.","family":"Fahmy","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Ain Shams University  Cairo Abbassia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6548-7823","authenticated-orcid":false,"given":"Gokhan","family":"Zengin","sequence":"additional","affiliation":[{"name":"Physiology and Biochemistry Laboratory, Department of Biology, Science Faculty Selcuk University  Konya Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sakina","family":"Yagi","sequence":"additional","affiliation":[{"name":"Department of Botany, Faculty of Science University of Khartoum  Khartoum Sudan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdullahi Ibrahim","family":"Uba","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Istanbul AREL University  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0972-0560","authenticated-orcid":false,"given":"Omayma A.","family":"Eldahshan","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy Ain Shams University  Cairo Abbassia Egypt"},{"name":"Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Centre of Drug Discovery Research and Development Ain Shams University  Cairo Abbassia Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ismail","family":"Koyuncu","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry, Faculty of Medicine Harran University  Sanliurfa Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ebru","family":"Temiz","sequence":"additional","affiliation":[{"name":"Program of Medical Promotion and Marketing, Health Services Vocational School Harran University  Sanliurfa Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Dall'Acqua","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Sut","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical and Pharmacological Sciences University of Padova  Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selami","family":"Selvi","sequence":"additional","affiliation":[{"name":"Department of Plant and Animal Production, Alt\u0131noluk Vocational School Bal\u0131kesir University  Bal\u0131kesir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-20412-3"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12091785"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fct.2022.113146"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27248979"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.103580"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/pr10101911"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/fst.43121"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.128403"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114205"},{"issue":"2","key":"e_1_2_6_11_1","first-page":"93","volume":"6","author":"Ghasemi Pirbalouti A.","year":"2015","journal-title":"J. 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