{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T06:17:41Z","timestamp":1764656261934,"version":"3.41.2"},"reference-count":34,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T00:00:00Z","timestamp":1702512000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The study aimed to analyze the effects of <jats:italic>Dendrobium<\/jats:italic> polysaccharides on the cough and airway reactivity and compare them with the effects of clinically used antitussives (codeine phosphate and butamirate citrate) and bronchodilators (salbutamol), using the guinea pig test system. <jats:italic>Dendrobium officinale<\/jats:italic> polysaccharides contained proteins (4.0\u2009wt%) and phenolic compounds (1.7\u2009wt%) with a molecular weight of 25,000\u2009g\/mol. The sugar analysis revealed a dominance of glucose (93.7\u2009wt%) and a lesser amount of mannose (5.1\u2009wt%) while other sugar quantities were negligible. Methylation analysis indicated the presence of highly branched polysaccharides. Glucose was found mainly as terminal, 1,4\u2010 and 1,6\u2010linked. Furthermore, some 1,4\u2010 and 1,6\u2010linked glucose units were found branched at O2, O3, and O6\/O4. Mannose was terminal and 1,4\u2010linked. NMR spectra signals indicate the presence of the (1\u21924)\u2010linked \u03b1\u2010<jats:sc>d<\/jats:sc>\u2010glucan, (1\u21924)\u2010linked \u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucan branched at position O6, (1\u21926)\u2010linked \u03b2\u2010<jats:sc>d<\/jats:sc>\u2010glucan branched at position O3 and (1\u21924)\u2010linked glucomannan. Pharmacological studies showed statistically significant antitussive activity of <jats:italic>Dendrobium<\/jats:italic> polysaccharides, exceeding the effect of clinically used antitussives, which may be partially associated with confirmed bronchodilation and the ability of polysaccharides to increase the threshold of cough receptor activation. <jats:italic>Dendrobium<\/jats:italic> polysaccharides may increase the possibility of symptomatic treatment of cough, especially in asthmatics.<\/jats:p>","DOI":"10.1002\/ardp.202300537","type":"journal-article","created":{"date-parts":[[2023,12,14]],"date-time":"2023-12-14T23:42:32Z","timestamp":1702597352000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Dendrobium officinale<\/i> polysaccharides\u2014chemical properties and pharmacodynamic effects on the airways in experimental conditions"],"prefix":"10.1002","volume":"357","author":[{"given":"Martina","family":"\u0160utovsk\u00e1","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Jessenius Faculty of Medicine Comenius University Martin Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jozef","family":"Ma\u017eerik","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Jessenius Faculty of Medicine Comenius University Martin Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaela","family":"Kocm\u00e1lov\u00e1","sequence":"additional","affiliation":[{"name":"Biomedical Centre BioMed, Jessenius Faculty of Medicine Comenius University Martin Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iveta","family":"Uhliarikov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1ria","family":"Matulov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0653-6012","authenticated-orcid":false,"given":"Peter","family":"Capek","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,12,14]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7436259"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(20)30001-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.06.156"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0008-6215(84)85242-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2012.08.045"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fshw.2022.07.051"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2010.09.015"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.03.003"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2016.11.048"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2021.118939"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2014.07.080"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2012.12.003"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2014.05.011"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2020.116326"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jff.2019.04.013"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1745-9974-1-2"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00862.2005"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2006.11.001"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2015.1069255"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.128.1.355"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000093932"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2018.06.073"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/168167a0"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(73)90377-1"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(99)99017-1"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/an9820700307"},{"key":"e_1_2_7_29_1","first-page":"1","volume":"8","author":"Jansson P. 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