{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T23:15:01Z","timestamp":1793315701189,"version":"4.5.0"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T00:00:00Z","timestamp":1705881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Nonalcoholic fatty liver disease (NAFLD) is a common liver disorder affecting a quarter of the global residents. Progression of NAFL into nonalcoholic steatohepatitis (NASH) may cause cirrhosis, liver cancer, and failure. Gut microbiota imbalance causes microbial components translocation into the circulation, triggering liver inflammation and NASH\u2010related fibrosis. MicroRNAs (miRNAs) regulate gene expression\n                    <jats:italic>via<\/jats:italic>\n                    repressing target genes. Exosomal miRNAs are diagnostic and prognostic biomarkers for NAFL and NASH liver damage. Our work investigated the role of the gut microbiota in NAFLD pathogenesis\n                    <jats:italic>via<\/jats:italic>\n                    the lipopolysaccharide\/toll\u2010like receptor 4\/Forkhead box protein O3 (LPS\/TLR\u20104\/FoxO3) pathway and certain miRNAs as noninvasive biomarkers for NAFL or its development to NASH. miRNA\u00a0expression levels were measured using quantitative reverse transcription polymerase chain reaction (qRT\u2010PCR) in 50 NAFL patients, 50 NASH patients, and 50 normal controls. Plasma LPS, TLR\u20104, adiponectin, peroxisome proliferator\u2010activated receptor \u03b3 (PPAR\u2010\u03b3), and FoxO3 concentrations were measured using enzyme\u2010linked immunosorbent assay (ELISA). In NAFL and NASH patients, miR\u2010122, miR\u2010128, FoxO3, TLR\u20104, LPS, and PPAR\u2010\u03b3 were upregulated while miR\u2010200, miR\u2010298, miR\u2010342, and adiponectin were downregulated compared with the normal\u00a0control. The examined miRNAs might distinguish NAFL and NASH patients from the normal control\u00a0using receiver operating characteristic analysis. Our study is the first to examine these miRNAs in NAFLD. Our findings imply that these are potentially promising biomarkers for noninvasive early NAFL diagnosis and NASH progression. Understanding the LPS\/TLR\u20104\/FoxO3 pathway involvement in NAFL\/NASH pathogenesis may aid disease management.\n                  <\/jats:p>","DOI":"10.1002\/ardp.202300631","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T09:03:38Z","timestamp":1705914218000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Exosomal miR\u2010122, miR\u2010128, miR\u2010200, miR\u2010298, and miR\u2010342 as novel diagnostic biomarkers in NAFL\/NASH: Impact of LPS\/TLR\u20104\/FoxO3 pathway"],"prefix":"10.1002","volume":"357","author":[{"given":"Ahmed M.","family":"Samy","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Nahda University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Kandeil","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine Beni\u2010Suef University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina","family":"Sabry","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Cairo University Cairo Egypt"},{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Badr University in Cairo Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"Abdel\u2010Ghany","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Nahda University Beni\u2010Suef Egypt"},{"name":"Department of Biochemistry, Faculty of Pharmacy Al\u2010Azhar University Assuit branch Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6737-8142","authenticated-orcid":false,"given":"Mohamed O.","family":"Mahmoud","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Beni\u2010Suef University Beni\u2010Suef Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","unstructured":"H.Boppidi Postgrad. 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