{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T14:15:01Z","timestamp":1787235301562,"version":"build-2736575974"},"reference-count":59,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T00:00:00Z","timestamp":1710460800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Influenza A virus (IAV) is a highly contagious respiratory pathogen that significantly threatens global health by causing seasonal epidemics and occasional, unpredictable pandemics. To identify new compounds with therapeutic potential against IAV, we designed and synthesized a series of 4\u2032\u2010morpholinodiazenyl chalcones using the molecular hybridization method, performed a high\u2010content screen against IAV, and found that (\n                    <jats:italic>E<\/jats:italic>\n                    )\u20101\u2010{4\u2010[(\n                    <jats:italic>E<\/jats:italic>\n                    )\u2010morpholinodiazenyl]phenyl}\u20103\u2010(3,4,5\u2010trimethoxyphenyl)prop\u20102\u2010en\u20101\u2010one (MC\u201022) completely neutralized IAV infection. While MC\u201022 allowed IAV to successfully internalize into the cell and fuse at the acidic late endosomes, it prevented viral capsid uncoating and genome release. Since IAV majorly utilizes clathrin\u2010mediated endocytosis (CME) for cellular entry, we examined whether MC\u201022 had any effect on CME, using nonviral cargoes that enter cells via clathrin\u2010dependent or \u2010independent pathways. Although MC\u201022 showed no effect on the uptake of choleratoxin B, a cargo that enters cells majorly via the clathrin\u2010independent pathway, it significantly attenuated the clathrin\u2010dependent internalization of both epidermal growth factor and transferrin. Cell biological analyses revealed a marked increase in the size of early endosomes upon MC\u201022 treatment, indicating an endosomal trafficking\/maturation defect. This study reports the identification of MC\u201022 as a novel CME\u2010targeting, highly potent IAV entry inhibitor, which is expected to neutralize a broad spectrum of viruses that enter the host cells via CME.\n                  <\/jats:p>","DOI":"10.1002\/ardp.202300670","type":"journal-article","created":{"date-parts":[[2024,3,16]],"date-time":"2024-03-16T02:12:28Z","timestamp":1710555148000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Morpholinodiazenyl chalcone blocks influenza A virus capsid uncoating by perturbing the clathrin\u2010mediated vesicular trafficking pathway"],"prefix":"10.1002","volume":"357","author":[{"given":"Ahammed A.","family":"Thottasseri","sequence":"first","affiliation":[{"name":"Department of Chemistry Pondicherry University Puducherry 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