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The technique employs diclofenac potassium as the internal standard, guaranteeing dependable and precise outcomes. This study aimed to assess the impact of the suggested combination therapy on treating inflammation resulting from rheumatoid arthritis (RA) in a rat model. The procedure was performed using an Agilent series 1200 model HPLC apparatus. The chromatographic conditions consist of isocratic elution mode, C18 column with dimensions of 150\u2009mm\u2009\u00d7\u20094.6\u2009mm\u2009\u00d7\u20095\u2009\u00b5m, flow rate of 1.5\u2009mL\/min, wavelength of 230\u2009nm, temperature of 50\u00b0C, and injection volume of 10\u2009\u00b5L. The elution was performed using a mobile phase consisting of a phosphate buffer with a pH of 3.5 and acetonitrile in a ratio of 80:20\u2009v\/v. Calibration curves were conducted for TIZ and ETC within the 1\u201350\u2009\u00b5g\/mL range, demonstrating linear trends with <jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> values over 0.999. The effectiveness and eco\u2010friendliness of the proposed method were evaluated using eight separate environmentally conscious metrics. The addition of TIZ and ETC to arthritic rodents amplified these effects significantly. Furthermore, TIZ and ETC significantly reduced serum levels in arthritic rodents, and safety investigations revealed normal complete blood count, liver, and renal functions. TIZ and ETC appear to have antiarthritic, anti\u2010inflammatory, and safe combinations, making them viable future treatment options for RA that are also safe and efficacious. Following validation by United States Food and Drug Administration\u00a0(US\u2010FDA) rules, all goods met the criteria.<\/jats:p>","DOI":"10.1002\/ardp.202400025","type":"journal-article","created":{"date-parts":[[2024,4,22]],"date-time":"2024-04-22T02:19:30Z","timestamp":1713752370000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Novel RP\u2010HPLC method for estimation of a newly developed combination of tizanidine and etoricoxib in rat plasma: Eight criteria for greens evaluation"],"prefix":"10.1002","volume":"357","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7312-4800","authenticated-orcid":false,"given":"Eman A.","family":"Assirey","sequence":"first","affiliation":[{"name":"Department of Chemistry, College of Science Taibah University, Al\u2010Madinah Al\u2010Munawarah Madinah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3966-7024","authenticated-orcid":false,"given":"Hoda A.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Chemistry Department, Faculty of Science at Yanbu Taibah University Yanbu Saudi Arabia"},{"name":"Department of Chemistry, Faculty of Science Cairo University Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1143-6892","authenticated-orcid":false,"given":"Rawan","family":"AL\u2010Faze","sequence":"additional","affiliation":[{"name":"Department of Chemistry, College of Science Taibah University, Al\u2010Madinah Al\u2010Munawarah Madinah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8830-1171","authenticated-orcid":false,"given":"M. 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