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Metabolite profiling of VBM using HPLC\u2010PDA\u2010ESI\u2010MS identified 12 compounds, mainly iridoids, phenylpropanoids, and flavonoids, where verbascoside represents the major compound. Different biochemical parameters such as aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP), bilirubin, and total protein levels were used to assess liver functions. All the evaluated samples exhibited hepatoprotective potential, but VBM exhibited maximum activity and a notable decline in ALP (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05, significant), even better than the standard drug (silymarin). VBM significantly reduced the elevated ALT, AST, ALP, and total bilirubin. It also triggered a significant elevation in total proteins compared with diseased animals. This was further consolidated by histopathological studies. <jats:italic>Verbena bonariensis<\/jats:italic> L. could serve as a potent hepatoprotective agent and may alleviate liver ailments.<\/jats:p>","DOI":"10.1002\/ardp.202400055","type":"journal-article","created":{"date-parts":[[2024,4,12]],"date-time":"2024-04-12T17:09:34Z","timestamp":1712941774000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Metabolic profiling of <i>Verbena bonariensis<\/i> L. extract by LC\/MS and evaluation of the hepatoprotective potential with isoniazid\u2010 and rifampicin\u2010induced hepatotoxicity in rats"],"prefix":"10.1002","volume":"357","author":[{"given":"Saiqa","family":"Ishtiaq","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Punjab University College of Pharmacy University of the Punjab Lahore Pakistan"},{"name":"Emory College of Arts and Science Center for the Study of Human Health, Emory University Atlanta Georgia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saira","family":"Rehman","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Punjab University College of Pharmacy University of the Punjab Lahore Pakistan"},{"name":"Faculty of Pharmaceutical Sciences Lahore University of Biological and Applied Sciences Lahore Punjab Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sairah Hafeez","family":"Kamran","sequence":"additional","affiliation":[{"name":"Institute of Pharmacy, Faculty of Pharmaceutical and Allied Health Sciences Lahore College for Women University Lahore Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahid Mehmood","family":"Akhtar","sequence":"additional","affiliation":[{"name":"Department of Pathology Gujranwala Medical College Gujranwala Punjab Pakistan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2645-0021","authenticated-orcid":false,"given":"Mai","family":"Albaik","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Preparatory Year Program Batterjee Medical College Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3799-0581","authenticated-orcid":false,"given":"Sameh S.","family":"Elhady","sequence":"additional","affiliation":[{"name":"King Abdulaziz University Herbarium, Faculty of Science King Abdulaziz University Jeddah Saudi Arabia"},{"name":"Department of Biological Sciences Faculty of Science, King Abdulaziz University Jeddah Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,4,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.12047"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox8090412"},{"issue":"2","key":"e_1_2_10_4_1","first-page":"15","volume":"2","author":"Bhardwaj A.","year":"2011","journal-title":"J. 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