{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T17:32:43Z","timestamp":1790098363035,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T00:00:00Z","timestamp":1715040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Two novel series of hydrazinyl\u2010based benzenesulfonamides <jats:bold>9a<\/jats:bold>\u2013<jats:bold>j<\/jats:bold> and <jats:bold>10a<\/jats:bold>\u2013<jats:bold>j<\/jats:bold> were designed and synthesized using SLC\u20100111 as the lead molecule. The newly synthesized compounds were evaluated for their inhibitory activity against four different human carbonic anhydrase (hCA) isoforms I, II, IX, and XII. Both the series reported here were practically inactive against the off\u2010target isozyme hCA I. Notably, derivative <jats:bold>10a<\/jats:bold> exhibited superior potency (<jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> of 10.2\u2009nM) than acetazolamide (AAZ) against the cytosolic isoform hCA II. The hCA IX and XII isoforms implicated in tumor progression were effectively inhibited with <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub>s in the low nanomolar range of 20.5\u2013176.6\u2009nM and 6.0\u2013127.5\u2009nM, respectively. Compound <jats:bold>9g<\/jats:bold> emerged as the most potent and selective hCA IX and XII inhibitor with <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> of 20.5\u2009nM and <jats:italic>S<\/jats:italic><jats:sub>I<\/jats:sub> of 200.1, and <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> of 6.0\u2009nM and <jats:italic>S<\/jats:italic><jats:sub>I<\/jats:sub> of 683.7, respectively, over hCA I. Furthermore, six compounds (<jats:bold>9a<\/jats:bold>, <jats:bold>9h<\/jats:bold>, <jats:bold>10a<\/jats:bold>, <jats:bold>10g<\/jats:bold>, <jats:bold>10i<\/jats:bold>, and <jats:bold>10j<\/jats:bold>) exhibited significant inhibition toward hCA IX (<jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub>s\u2009=\u200927.0, 41.1, 27.4, 25.9, 40.7, and 30.8\u2009nM) relative to AAZ and SLC\u20100111 (<jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub>s\u2009=\u200925.0 and 45.0\u2009nM, respectively). These findings underscore the potential of these derivatives as potent and selective inhibitors of hCA IX and XII over the off\u2010target hCA I and II.<\/jats:p>","DOI":"10.1002\/ardp.202400157","type":"journal-article","created":{"date-parts":[[2024,5,7]],"date-time":"2024-05-07T20:43:46Z","timestamp":1715114626000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["In\u2010vitro and in\u2010silico investigations of SLC\u20100111 hydrazinyl analogs as human carbonic anhydrase I, II, IX, and XII inhibitors"],"prefix":"10.1002","volume":"357","author":[{"given":"Vikas","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Chemistry Kurukshetra University Kurukshetra Haryana India"},{"name":"Department of Chemistry Pt. 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