{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T17:53:05Z","timestamp":1755798785539,"version":"3.44.0"},"reference-count":61,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T00:00:00Z","timestamp":1724716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Tetrahydropyrimidine (compound <jats:bold>A<\/jats:bold>\u2009=\u2009methyl 4\u2010[4\u2032\u2010(heptyloxy)\u20103\u2032\u2010methoxyphenyl]\u20101,6\u2010dimethyl\u20102\u2010thioxo\u20101,2,3,4\u2010tetrahydropyrimidine\u20105\u2010carboxylate) was chosen for in vivo studies after exhibiting noteworthy in vitro activity against the K562 and MDA\u2010MB\u2010231 cell lines, with IC<jats:sub>50<\/jats:sub> values of 9.20\u2009\u00b1\u20090.14\u2009\u00b5M and 12.76\u2009\u00b1\u20091.93\u2009\u00b5M, respectively. According to experimental (fluorescence titration, viscosity, and differential scanning calorimetry) results, <jats:bold>A<\/jats:bold> interacts with DNA <jats:italic>via<\/jats:italic> the minor groove. In vivo, acute oral toxicity studies in Wistar albino rats proved no noticeable symptoms of either toxicity or death during the follow\u2010up period. Genotoxic and antigenotoxic studies at three different concentrations of <jats:bold>A<\/jats:bold> (5, 10, and 20\u2009mg\/kg of body weight) in Wistar albino rats showed that the dose of 5\u2009mg\/kg body weight did not cause DNA damage and had a remarkable DNA protective activity against CCl<jats:sub>4<\/jats:sub>\u2010induced DNA damage, with a percentage reduction of 78.7%. It is also important to note that, under the investigated concentrations of <jats:bold>A<\/jats:bold>, liver damage is not observed. Considering all experimental outcomes realized under various in vivo investigations (acute oral toxicity, genotoxicity, antigenotoxicity, and biochemical tests), compound <jats:bold>A<\/jats:bold> could be a promising candidate for further clinical testing.<\/jats:p>","DOI":"10.1002\/ardp.202400409","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T03:29:50Z","timestamp":1724729390000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["DNA interaction of selected tetrahydropyrimidine and its effects against CCl<sub>4<\/sub>\u2010induced hepatotoxicity in vivo: Part II"],"prefix":"10.1002","volume":"357","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4242-1562","authenticated-orcid":false,"given":"Emilija","family":"Milovi\u0107","sequence":"first","affiliation":[{"name":"Department of Sciences, Institute for Information Technologies Kragujevac University of Kragujevac 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