{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T10:49:11Z","timestamp":1784371751050,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study investigates aliphatic sulfonamide derivatives as inhibitors of the \u03b1\u2010, \u03b2\u2010, and \u03b3\u2010class carbonic anhydrase (CA) isoforms from <jats:italic>Vibrio cholerae<\/jats:italic> (VchCAs). A series of 26 compounds bearing a triazole linker and urea\u2010 or ether\u2010based tails were described and evaluated for their inhibitory action using a stopped\u2010flow CO<jats:sub>2<\/jats:sub> hydrase technique. These inhibitors demonstrated a preferential efficacy against VchCA\u03b2. Specifically, the ureido derivatives showed the highest inhibitory potency with inhibition constants (<jats:italic>K<\/jats:italic><jats:sub>I<\/jats:sub>s) in the submicromolar range (0.67\u20130.93\u2009\u00b5M). Selectivity indices were calculated to assess the selective inhibition of VchCA\u03b2 over human CA I and II, as well as other VchCA isozymes. Urea\u2010linked compounds demonstrated a significant 25\u2010 to 125\u2010fold selectivity for VchCA\u03b2 over hCAs and 14\u2010 to 26\u2010fold over other VchCAs. Molecular modeling elucidated the interactions contributing to the efficacy and selectivity of aliphatic sulfonamides as VchCA inhibitors, aligning with and reinforcing the experimental results. The latter suggests that aliphatic sulfonamides could serve as valid targeted therapeutics to treat <jats:italic>V. cholerae<\/jats:italic> infections.<\/jats:p>","DOI":"10.1002\/ardp.202400814","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T09:11:34Z","timestamp":1734426694000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Exploring aliphatic sulfonamides as multiclass inhibitors of the carbonic anhydrases from the pathogen bacterium <i>Vibrio cholerae<\/i>"],"prefix":"10.1002","volume":"358","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6874-9419","authenticated-orcid":false,"given":"Niccol\u00f2","family":"Paoletti","sequence":"first","affiliation":[{"name":"NEUROFARBA Department, Laboratory of Molecular Modelling, Cheminformatics &amp; QSAR University of Florence Firenze Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0669-7481","authenticated-orcid":false,"given":"Simone","family":"Giovannuzzi","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Pharmaceutical and Nutraceutical Section University of Florence Firenze Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5520-740X","authenticated-orcid":false,"given":"Alessandro","family":"Bonardi","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Laboratory of Molecular Modelling, Cheminformatics &amp; QSAR University of Florence Firenze Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"De Luca","sequence":"additional","affiliation":[{"name":"Department of Biology Agriculture and Food Sciences, National Research Council (CNR), Institute of Biosciences and Bioresources Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemente","family":"Capasso","sequence":"additional","affiliation":[{"name":"Department of Biology Agriculture and Food Sciences, National Research Council (CNR), Institute of Biosciences and Bioresources Naples Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessio","family":"Nocentini","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Pharmaceutical and Nutraceutical Section University of Florence Firenze Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Gratteri","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Laboratory of Molecular Modelling, Cheminformatics &amp; QSAR University of Florence Firenze Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Pharmaceutical and Nutraceutical Section University of Florence Firenze Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2023.1155751"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12050916"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed9050103"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0226549"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2019.06.031"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/msphere.00748-20"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-021-00985-w"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imj.2023.11.004"},{"key":"e_1_2_8_10_1","first-page":"325","volume":"38","author":"Levine M. 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