{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T18:14:40Z","timestamp":1792174480258,"version":"4.4.0"},"reference-count":44,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T00:00:00Z","timestamp":1742774400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    In this study, we propose identifying potential novel compounds targeting carbonic anhydrase (CA) IX and anticancer activity. To study the impact of these synthesized compounds on CA IX and anticancer activity, we have developed and synthesized novel benzimidazole\u2010hydrazone derivatives (\n                    <jats:bold>3a\u20133j<\/jats:bold>\n                    ). The target compounds'\n                    <jats:sup>1<\/jats:sup>\n                    H NMR (nuclear magnetic resonance),\n                    <jats:sup>13<\/jats:sup>\n                    C NMR, and high resolution mass spectrometry spectra were used to confirm their chemical structures. L929 (healthy mouse fibroblast cell line) used as control healthy cell line and MCF\u20107 (breast cancer), C6 (rat glioblastoma), HT\u201029 (colon cancer), cells were used in cell culture studies. As a result of cell culture studies, it was determined that the newly synthesized compounds\n                    <jats:bold>3d<\/jats:bold>\n                    and\n                    <jats:bold>3j<\/jats:bold>\n                    had cytotoxic effects on colon cancer. Again, it was determined that the compound\n                    <jats:bold>3d<\/jats:bold>\n                    had a more toxic effect than cisplatin on both breast cancer and glioma cells. According to the CA IX activity results, compounds\n                    <jats:bold>3d<\/jats:bold>\n                    and\n                    <jats:bold>3j<\/jats:bold>\n                    were found to have the highest activity. Compounds\n                    <jats:bold>3d<\/jats:bold>\n                    and\n                    <jats:bold>3j<\/jats:bold>\n                    are essential for having anti\u2010cancer properties and inhibiting the carbonic anhydrase IX enzyme. Molecular docking of these compounds was carried out in the active site of CA IX. Flow cytometry and immunofluorescence microscope analyses also confirmed that these compounds had cytotoxic effects on cancer cells.\n                  <\/jats:p>","DOI":"10.1002\/ardp.202400930","type":"journal-article","created":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T15:28:48Z","timestamp":1742830128000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["New benzimidazole derivatives containing hydrazone group as anticancer agents: Inhibition of carbonic anhydrase IX and molecular docking studies"],"prefix":"10.1002","volume":"358","author":[{"given":"Hayrani Eren","family":"Bostanc\u0131","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Faculty of Pharmacy Cumhuriyet University  Sivas Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet Taha","family":"Y\u0131ld\u0131z","sequence":"additional","affiliation":[{"name":"Hamidiye Faculty of Health Sciences University of Health Sciences  Istanbul Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serkan","family":"Kapanc\u0131k","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine Cumhuriyet University  Sivas Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeynep Deniz","family":"\u015eahin Inan","sequence":"additional","affiliation":[{"name":"Department of Histology and Embryology Sivas Cumhuriyet University  Sivas Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hac\u0131 Ahmet","family":"K\u0131l\u0131\u00e7","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zen","family":"\u00d6zensoy G\u00fcler","sequence":"additional","affiliation":[{"name":"Department of Medical Biology, Faculty of Medicine Ankara Yildirim Beyazit University  Ankara Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1879-1034","authenticated-orcid":false,"given":"Ulviye","family":"Acar \u00c7evik","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Anadolu University  Eski\u015fehir Turkey"},{"name":"Medicinal Plant, Drug and Scientific Research and Application Center (AUBIBAM) Anadolu University  Eskisehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8815-153X","authenticated-orcid":false,"given":"Yusuf","family":"\u00d6zkay","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Anadolu University  Eski\u015fehir Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2252-0923","authenticated-orcid":false,"given":"Zafer As\u0131m","family":"Kaplanc\u0131kl\u0131","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Faculty of Pharmacy Anadolu University  Eski\u015fehir Turkey"},{"name":"The Rectorate of Bilecik \u015eeyh Edebali University  Bilecik Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.cbi.2024.110947","volume":"393","author":"Lv Q.","year":"2024","journal-title":"Chem. 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