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In this study, we identified lithospermic acid (LTS0059529) from <jats:italic>Salvia miltiorrhiza<\/jats:italic> as a potential dual inhibitor of pancreatic lipase (PL) and human carbonic anhydrase VA (<jats:italic>h<\/jats:italic>CA VA), two key enzymes in lipid metabolism. Using molecular docking and dynamics simulations, we observed that lithospermic acid interacts with Zn\u00b2\u207a in <jats:italic>h<\/jats:italic>CA VA via its benzofuran carboxylate moiety and forms stable complexes with PL through hydrogen bonding with ASP 205 and \u03c0\u2013stacking interactions with PHE 77 and PHE 215. Experimental validation confirmed its inhibitory activity, with <jats:italic>K<\/jats:italic><jats:sub>i<\/jats:sub> values of 33.1\u2009\u00b1\u20091.6\u2009\u03bcM for PL and 0.69\u2009\u00b1\u20090.01\u2009\u03bcM for <jats:italic>h<\/jats:italic>CA VA. While its inhibition of <jats:italic>h<\/jats:italic>CA VA is not isoform\u2010specific, lithospermic acid demonstrates significant potential as a dual inhibitor, targeting complementary pathways in obesity management. This study is the first to explore its dual action on PL and <jats:italic>h<\/jats:italic>CA VA, highlighting a promising strategy for future antiobesity therapies. Further research will focus on optimizing selectivity and potency to develop safer and more effective treatments.<\/jats:p>","DOI":"10.1002\/ardp.202500046","type":"journal-article","created":{"date-parts":[[2025,4,21]],"date-time":"2025-04-21T14:14:26Z","timestamp":1745244866000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Discover the Power of Lithospermic Acid as Human Carbonic Anhydrase VA and Pancreatic Lipase Inhibitor Through In Silico and In Vitro Studies"],"prefix":"10.1002","volume":"358","author":[{"given":"Emanuele Liborio","family":"Citriniti","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze della Salute Universit\u00e0 \u201cMagna Gr\u00e6cia\u201d di Catanzaro Catanzaro Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0680-7097","authenticated-orcid":false,"given":"Roberta","family":"Rocca","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze della Salute Universit\u00e0 \u201cMagna Gr\u00e6cia\u201d di Catanzaro Catanzaro Italy"},{"name":"Net4Science S.r.l. 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