{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:50:05Z","timestamp":1787169005537,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Understanding how multidrug\u2010resistant (MDR) bacteria and fungi defy the existing antimicrobial agents requires innovative tools and techniques for real\u2010time, in situ exploration of bacterial responses to antibiotics. Fluorescence\u2010tagged antibiotics or dyes with inherent antimicrobial activity can provide a profound understanding of the molecular biology underlying antibiotic action and resistance mechanisms. Cutting\u2010edge research highlights the pursuit of benzo\u2010\u03b1\u2010pyrone (coumarin) derivatives due to their excellent pharmacokinetics, diverse pharmacological activities, and innovative fluorescence molecular probes. In this study, donor\u2010\u03c0\u2010acceptor\u2010based coumarin dyes were designed and evaluated for antimicrobial efficacy against fungal strains (\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    ), Gram\u2010negative pathogens (\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ), and Gram\u2010positive bacteria (\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ). I\u20106 exhibited notable antimicrobial activity against\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and\n                    <jats:italic>C. albicans<\/jats:italic>\n                    compared with\n                    <jats:italic>E. coli<\/jats:italic>\n                    . Conversely, I\u20109, a congener of I\u20106, showed a comparable affinity for\n                    <jats:italic>S. aureus<\/jats:italic>\n                    but found poor activity against the remaining tested strains. Mechanistic investigative studies unveiled that the inhibitory efficacy of I\u20106 can be attributed to its capacity to generate high reactive oxygen species (ROS) formation. Despite the evident antimicrobial potential of I\u20106 in the data, our future prospects, including real\u2010time visualization to study physiological processes like uptake, distribution, and mechanism of action through fluorescence\u2010based imaging modalities, could enhance the applicability of these probes.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70032","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T05:46:16Z","timestamp":1751348776000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["New D\u2010\u03c0\u2010A\u2010Based Coumarin\u2010 Derived Fluorescent Theranostic Probes With Broad\u2010Spectrum Antimicrobial Activity"],"prefix":"10.1002","volume":"358","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5615-5058","authenticated-orcid":false,"given":"Himanshu","family":"Rai","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Engineering &amp; Technology Indian Institute of Technology (BHU) Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3188-3566","authenticated-orcid":false,"given":"Atul Kumar","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Department of Chemistry Indian Institute of Technology (BHU) Varanasi India"},{"name":"School of Applied &amp; Life Sciences Uttaranchal University Dehradun Uttarakhand India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0399-8505","authenticated-orcid":false,"given":"Aishwarya","family":"Nikhil","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Medical Sciences Banaras Hindu University Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ankit","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Engineering &amp; Technology Indian Institute of Technology (BHU) Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prahalad Singh","family":"Bharti","sequence":"additional","affiliation":[{"name":"Department of Biophysics All India Institute of Medical Sciences  New Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh Kumar","family":"Maury","sequence":"additional","affiliation":[{"name":"Department of Chemistry Indian Institute of Technology (BHU) Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Munesh Kumar","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Microbiology Institute of Medical Sciences Banaras Hindu University Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sundaram","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Chemistry Indian Institute of Technology (BHU) Varanasi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7029-1163","authenticated-orcid":false,"given":"Saroj","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Biophysics All India Institute of Medical Sciences  New Delhi India"},{"name":"Department of Health, Education and Technology Lulea University of Technology Lulea Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8003-5566","authenticated-orcid":false,"given":"Gyan","family":"Modi","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Engineering &amp; 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