{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T08:10:10Z","timestamp":1763021410191,"version":"3.45.0"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To filter the active components of Fuzi Lizhong decoction (FLD) treating gastric ulcer (GU), compound information of herbs making up FLD was collected. Components in FLD extract were identified using ultrahigh performance liquid chromatography coupled with quadrupole\u2010exactive orbitrap mass spectrometry (UHPLC\/Q\u2010Exactive MS). After that, network pharmacology and molecular docking were applied for predicting active components. Next, GES\u20101 cell survival rate experiments were conducted to verify the active components. At last, real\u2010time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT\u2010qPCR) was applied to confirm active components. Results showed that a total of 58 components in FLD were qualified and 55 of them functioned on 310 GU\u2010relevant targets, involved in 154 signaling pathways. Through molecular docking of the top 30 components with the top 30 targets and referring to the reference, 9 components and their targets were predicted to be active components. Among them, atractylenolide II, tyrosine, and atractylenolide III were verified to inhibit GES\u20101 cell apoptosis, confirmed to reverse the gene expression of GU\u2010relevant targets involving\n                    <jats:italic>PPARG<\/jats:italic>\n                    ,\n                    <jats:italic>EGFR<\/jats:italic>\n                    ,\n                    <jats:italic>MAPK1<\/jats:italic>\n                    , and\n                    <jats:italic>ESR1<\/jats:italic>\n                    in GES\u20101 cells. So, atractylenolide II, tyrosine, and atractylenolide III were considered as active components of FLD treating GU. In conclusion, active components of FLD treating GU were filtered by applying UHPLC\/Q\u2010Exactive MS, uniting network pharmacology with the assistance of molecular docking and RT\u2010qPCR. FLD might exert anti\u2010GU effect through atractylenolide II, tyrosine, and atractylenolide III functioning as the relevant targets.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70036","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T15:26:54Z","timestamp":1752074814000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Filtering of Active Components of Fuzi Lizhong Decoction on Gastric Ulcer by UHPLC\/Q\u2010Exactive MS Uniting Network Pharmacology"],"prefix":"10.1002","volume":"358","author":[{"given":"Haijun","family":"Wang","sequence":"first","affiliation":[{"name":"School of General Medicine and Continuing Education Qiqihar Medical University Qiqihar China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Qiqihar University Qiqihar China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaichen","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Qiqihar University Qiqihar China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qingzhu","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Science and Agroforestry Qiqihar University Qiqihar China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Technology Innovation Center of Industrial Hemp State Administration for Market Regulation Qiqihar China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0760-7676","authenticated-orcid":false,"given":"Yang","family":"Xin","sequence":"additional","affiliation":[{"name":"College of Chemistry and Chemical Engineering Qiqihar University Qiqihar China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2013.12.001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10337-017-3308-0"},{"key":"e_1_2_8_4_1","first-page":"86","article-title":"The Influence of Fuzi Lizhong Decoction Combined With Acupoint Warm Moxibustion and Western Medicine on Inflammatory Factors and Gastric Mucosal Barrier Function in Patients With Spleen\u2010Stomach Cold Deficiency Type Stomach Ulcer","volume":"17","author":"Pei L.","year":"2024","journal-title":"Chinese Journal of Clinical Rational Drug Use"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8774913"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.2174\/0113816128308565240710114350"},{"key":"e_1_2_8_7_1","first-page":"297","article-title":"Integrating Network Pharmacology and Experimental Models to Investigate the Efficacy of QYHJ on Pancreatic Cancer","volume":"393","author":"Yang P. 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