{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T08:10:43Z","timestamp":1763021443087,"version":"3.45.0"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T00:00:00Z","timestamp":1752192000000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Biofilms pose significant challenges in multiple settings due to their resistance to conventional treatments. In this study, we designed and synthesized a novel class of nature\u2010inspired 5,7\u2010dihydroxy\u20102,2\u2010dimethylchroman\u20104\u2010one derivatives as binders of WrbA, a potential target for biofilm modulation. Using a structure\u2010based computational approach, a small library of analogs with varied amide moieties was developed and synthesized. The evaluation of their binding affinity to WrbA demonstrated good\u2010to\u2010excellent\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d<\/jats:sub>\n                    values, as confirmed by microscale thermophoresis (MST). Antibiofilm assays against\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    and\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    revealed different modulating effects on biofilm formation, conceivably linked to ROS production. These findings emphasize the importance of ROS levels in biofilm, as well as the pivotal role of WrbA as a target in its regulation.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70049","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T11:05:21Z","timestamp":1752231921000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nature\u2010Inspired Compounds Targeting\n                    <i>Escherichia coli<\/i>\n                    WrbA as Biofilm\u2010Modulating Agents: Computational Design, Synthesis, and Biological Evaluation"],"prefix":"10.1002","volume":"358","author":[{"given":"Matteo","family":"Mori","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences University of Milan Milano Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6282-4273","authenticated-orcid":false,"given":"Enrico Mario 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