{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T02:04:36Z","timestamp":1791511476526,"version":"4.3.4"},"reference-count":54,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":28,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Inspired by the anti\u2010breast cancer and anti\u2010tubulin potential of coumarin, indole, chalcone, and isatin moieties, a new series of triazole\u2010tethered coumarin\u2010fused chalcone and isatin hybrids were designed, synthesized, and evaluated for their anti\u2010breast cancer activities. Among the series of hybrid compounds, JKUB2 showed the strongest activity against MCF\u20107 breast cancer cells with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 1.28\u2009\u00b5M. JKUB2 exhibited tubulin polymerization inhibition potential (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009=\u20091.31\u2009\u00b5M) and induced apoptosis in breast cancer cells by arresting the cell cycle at the G2\/M phase. Apart from that, JKUB2 showed higher selectivity (selectivity index: 6.36) toward MCF\u20107 cells over normal skin fibroblast cells (L929). Morphological studies further confirmed the capability of JKUB2 to induce cell death via apoptotic pathways. Molecular docking and dynamics simulations studies confirmed the desired interactions of JKUB2 in the colchicine binding site of microtubules, responsible for their polymerization inhibition. Overall, the study represents JKUB2 as a potential anti\u2010breast cancer agent that acts via tubulin polymerization inhibition and induces cell death by apoptotic pathways, and warrants further research as well as acts as an effective hit lead for further development of potent and safer anti\u2010breast cancer agents.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70060","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T07:06:27Z","timestamp":1753772787000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Design, Synthesis, and Biological Evaluation of Triazole Tethered Coumarin\u2010Indole Fused Chalcone\u2010Isatin Derivatives as a New Class of Anti\u2010Breast Cancer Agents"],"prefix":"10.1002","volume":"358","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7208-8396","authenticated-orcid":false,"given":"Jasreen Kaur","family":"Uppal","sequence":"first","affiliation":[{"name":"University Institute of Pharma Sciences Chandigarh University Mohali India"},{"name":"Khalsa College of Pharmacy Amritsar Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1905-0986","authenticated-orcid":false,"given":"Rajiv","family":"Sharma","sequence":"additional","affiliation":[{"name":"University Institute of Pharma Sciences Chandigarh University Mohali India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13174287"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21754"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.5306\/wjco.v13.i3.209"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-025-03502-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2022.102339"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2023.107078"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hsr.2023.100119"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2017.12.012"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2020.08.015"},{"issue":"7","key":"e_1_2_8_11_1","first-page":"2920","article-title":"Breast Cancer: Molecular Mechanisms of Underlying Resistance and Therapeutic Approaches","volume":"12","author":"Tufail M.","year":"2022","journal-title":"American Journal of Cancer Research"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1317"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(97)80081-6"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/b403634d"},{"issue":"4","key":"e_1_2_8_15_1","first-page":"816","article-title":"Mitotic Block Induced in Hela Cells by Low Concentrations of Paclitaxel (Taxol) Results in Abnormal Mitotic Exit and Apoptotic Cell Death","volume":"56","author":"Jordan M. 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