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All compounds were characterized using various spectroscopic techniques. Single\u2010crystal XRD analysis was performed to determine the molecular structure of two compounds. The newly synthesized compounds exhibited significant inhibitory effects against acetylcholinesterase (AChE) and human carbonic anhydrase isoforms I and II (hCA I and hCA II) enzymes. These compounds demonstrated promising inhibition profiles, with\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>i<\/jats:sub>\n                    values ranging from 12.4\u2009\u00b1\u20095.4 to 109.4\u2009\u00b1\u200949.9\u2009nM for hCA I, 23.1\u2009\u00b1\u200911.2 to 115.0\u2009\u00b1\u200917.9\u2009nM for hCA II, and 0.7\u2009\u00b1\u20090.3 to 4.4\u2009\u00b1\u20091.0\u2009nM for AChE. In comparison, the reference compound acetazolamide showed\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>i<\/jats:sub>\n                    values of 30.5\u2009\u00b1\u20096.7\u2009nM and 37.4\u2009\u00b1\u20097.8\u2009nM against hCA I and hCA II isoenzymes, respectively. Additionally, tacrine, a known AChE inhibitor, exhibited a\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>i<\/jats:sub>\n                    value of 5.1\u2009\u00b1\u20092.7\u2009nM. The dual inhibition of CA and AChE represents a valuable pharmacological approach with a wide range of therapeutic applications. The explanation and evaluation of the enzyme inhibition data obtained in line with the interactions of the synthesized compounds with hCA I, hCA II, and AChE enzymes were carried out by molecular docking studies. In particular, we focused on the three compounds (\n                    <jats:bold>4e<\/jats:bold>\n                    ,\n                    <jats:bold>4f<\/jats:bold>\n                    , and\n                    <jats:bold>4j<\/jats:bold>\n                    for hCA I;\n                    <jats:bold>3g<\/jats:bold>\n                    ,\n                    <jats:bold>4f<\/jats:bold>\n                    , and\n                    <jats:bold>4k<\/jats:bold>\n                    for hCA II; and\n                    <jats:bold>4e<\/jats:bold>\n                    ,\n                    <jats:bold>4f<\/jats:bold>\n                    ,\n                    <jats:bold>4j<\/jats:bold>\n                    , and\n                    <jats:bold>4l<\/jats:bold>\n                    for AChE) with the highest potential activity with each enzyme. The physicochemical, ADME, drug\u2010likeness, medicinal chemistry, and toxicity properties of the potential ligands were then predicted so that their drug candidate suitability for further studies is revealed.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70063","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T05:03:06Z","timestamp":1753851786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["5(6)\u2010Benzoyl\u2010Substituted Benzimidazoles and Their Benzimidazolium Salts: Design, Synthesis, Characterization, Crystal Structure, and Some Metabolic Enzymes Inhibition Properties"],"prefix":"10.1002","volume":"358","author":[{"given":"Ayd\u0131n","family":"Akta\u015f","sequence":"first","affiliation":[{"name":"Inonu University, Vocational School of Health Service Malatya 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Gaziantep University Gaziantep T\u00fcrkiye"},{"name":"Ulu\u011f Bey High Technology Application and Research Center (ULUTEM) Gaziantep University Gaziantep T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Funda Sultan","family":"Ekti","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Science Eski\u015fehir Technical University Eski\u015fehir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5993-1668","authenticated-orcid":false,"given":"\u0130lhami","family":"G\u00fcl\u00e7in","sequence":"additional","affiliation":[{"name":"Faculty of Science, Department of Chemistry Atat\u00fcrk University Erzurum T\u00fcrkiye"},{"name":"Rectorate of A\u011fr\u0131 \u0130brahim \u00c7e\u00e7en University A\u011fr\u0131 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