{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T20:23:42Z","timestamp":1789503822068,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T00:00:00Z","timestamp":1759449600000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    3\u2010Deoxysappanchalcone (3\u2010DSC), extracted from\n                    <jats:italic>Caesalpinia sappan<\/jats:italic>\n                    L., is recognized for its anti\u2010inflammatory, anti\u2010influenza, and anti\u2010allergic effects, but its antithrombotic properties have not been investigated. This study explores whether 3\u2010DSC exhibits antithrombotic effects and examines the mechanisms involved. The antithrombotic properties of 3\u2010DSC were assessed through various methods, including tests for clotting times, platelet aggregation analysis, evaluation of factor Xa activity and production, nitric oxide levels, and the expression of related proteins. This study found that 3\u2010DSC extended the clotting time in human platelet\u2010poor plasma at levels comparable to rivaroxaban, a standard anticoagulant, and reduced platelet aggregation triggered by ADP or the thromboxane A2 analog U46619. Additionally, 3\u2010DSC suppressed the phosphorylation of PLC\u03b32 and PKC, as well as intracellular calcium release, which are essential for platelet aggregation. It also decreased the expression of adhesion molecules P\u2010selectin and PAC\u20101. Furthermore, 3\u2010DSC promoted nitric oxide production while reducing endothelin\u20101 secretion in endothelial cells exposed to ADP or U46619. Lastly, it inhibited both the activity and production of coagulation factor Xa in endothelial cells and prevented activated factor X (FXa)\u2010induced platelet aggregation. Injection of 3\u2010DSC significantly shortened the time required for thrombus resolution, reduced the size and number of thrombi, and decreased mortality in mouse models of thromboembolism. The study demonstrates that 3\u2010DSC effectively exhibits antithrombotic activity by prolonging the clotting time, inhibiting platelet aggregation, and reducing factor Xa activity, comparable to standard anticoagulants. These findings highlight the potential of 3\u2010DSC as a promising therapeutic agent targeting multiple pathways involved in thrombosis, with reduced side effects.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70101","type":"journal-article","created":{"date-parts":[[2025,10,3]],"date-time":"2025-10-03T13:49:52Z","timestamp":1759499392000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Inhibitory Effects of 3\u2010Deoxysappanchalcone on Factor Xa Activity, Platelet Aggregation, and Experimentally Induced Thrombosis"],"prefix":"10.1002","volume":"358","author":[{"given":"Jinhee","family":"Lee","sequence":"first","affiliation":[{"name":"College of Pharmacy, CMRI, Research Institute of Pharmaceutical Sciences Kyungpook National University Daegu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gyuri","family":"Han","sequence":"additional","affiliation":[{"name":"College of 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