{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:40:15Z","timestamp":1789094415226,"version":"build-2803163510"},"reference-count":62,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T00:00:00Z","timestamp":1758931200000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    This study aimed to improve the drug\u2010like properties of benzimidazole\u2010based Pt(II)\u2010N\u2010heterocyclic carbene (NHC) complexes, particularly by enhancing their water solubility and delivery to cancer cells. Accordingly, four new Pt(II) complexes of the benzimidazol\u20102\u2010ylidene type, featuring monodentate carboxylato ligands, were prepared and their structures confirmed through a combination of spectroscopic and crystallographic techniques. Their stability in aqueous solution and cell culture medium was investigated by\n                    <jats:sup>1<\/jats:sup>\n                    H NMR spectroscopy and HPLC\u2010MS analysis. Cytotoxicity was assessed using the MTT assay in ovarian cancer cell lines (A2780wt (cisplatin sensitive) and A2780cis (cisplatin resistant)) and a noncancerous bone marrow stromal cell line (HS\u20105). Most complexes exhibited cytotoxicity comparable to or exceeding that of carboplatin, with preferential activity toward cancer cells. Loading of all four Pt(II) complexes into bacterial ghost cells (BGs) derived from two different nonpathogenic bacterial strains,\n                    <jats:italic>Escherichia coli (E. coli)<\/jats:italic>\n                    Nissle 1917 and\n                    <jats:italic>E. coli<\/jats:italic>\n                    NM522 notably enhanced the intracellular accumulation and cytotoxicity. Furthermore, mechanistic studies demonstrated that all tested compounds, regardless of formulation, induced apoptosis. Their potential to trigger immunogenic cell death was also evaluated, though only a modest effect was observed on selected hallmarks. Collectively, these findings highlight the potential of dicarboxylatoplatinum(II)\u2010NHC complexes, particularly loaded into BG\u2010based formulations, as promising anticancer drug candidates.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70108","type":"journal-article","created":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T12:10:09Z","timestamp":1758975009000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Loading of Dicarboxylatoplatinum(II)\u2010NHC Complexes in Bacterial Ghosts as an Advanced Development in Cancer Therapy"],"prefix":"10.1002","volume":"358","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-0054-524X","authenticated-orcid":false,"given":"Amelie","family":"Scherfler","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, Institute of Pharmacy University of Innsbruck Innsbruck 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