{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:17:28Z","timestamp":1772997448500,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T00:00:00Z","timestamp":1764806400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The rise of multidrug\u2010resistant pathogens, including MRSA, underscores the urgent need for novel antibacterial agents with minimum resistance potential and enhanced potency. Herein, we report the design and synthesis of novel phenylvinylsulfonate\u2010anchored azetidin\u20102\u2010ones\n                    <jats:bold>8a\u2013h<\/jats:bold>\n                    &amp;\n                    <jats:bold>9i\u2013j<\/jats:bold>\n                    via the reaction of (\n                    <jats:italic>E<\/jats:italic>\n                    )\u20102\u2010phenylethene\u20101\u2010sulfonyl chloride with differently substituted 3\u2010hydroxy azetidin\u20102\u2010ones. Structural confirmation was achieved by\n                    <jats:sup>1<\/jats:sup>\n                    H NMR,\n                    <jats:sup>13<\/jats:sup>\n                    C NMR, HRMS, and stereochemical elucidation based on the\n                    <jats:italic>J\u2010<\/jats:italic>\n                    coupling values of\n                    <jats:italic>C<\/jats:italic>\n                    3\u2013H and\n                    <jats:italic>C<\/jats:italic>\n                    4\u2013H. Among the series, compound\n                    <jats:bold>9j<\/jats:bold>\n                    exhibited the most potent antibacterial activity against MRSA (MIC: 7.5\u2009\u03bcg\/mL), outperforming Tetracycline. Mechanistic investigation using ROS quantification (DCFH\u2010DA assay) revealed markedly elevated intracellular ROS (OD: 71,467), suggesting a possible ROS\u2010mediated bacterial damage mechanism. Molecular docking interactions further supported the strong binding of\n                    <jats:bold>9j<\/jats:bold>\n                    to penicillin\u2010binding protein 2a (PBP2a, PDB: 1VQQ) with a docking score of \u20136.68\u2009kcal\/mol. Additionally,\n                    <jats:bold>9j<\/jats:bold>\n                    exhibited selective cytotoxicity toward HCT\u2010116 colon cancer cells with an IC\n                    <jats:sub>50<\/jats:sub>\n                    value of 9.82\u2009\u00b1\u20090.21\u2009\u03bcM while maintaining moderate viability (~52%) in NIH\u20103T3 normal fibroblast cells. Collectively, these findings highlight phenylvinylsulfonate\u2010anchored azetidin\u20102\u2010ones as promising antibacterial agents with a ROS\u2010driven mechanism and supplementary selective cytotoxic potential.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70163","type":"journal-article","created":{"date-parts":[[2025,12,4]],"date-time":"2025-12-04T08:03:06Z","timestamp":1764835386000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenylvinylsulfonate\u2010Anchored Azetidin\u20102\u2010ones as Potent Antibacterial Agents: ROS\u2010Mediated Mechanism, Selective Cytotoxicity, and In Silico Target\u2010Interaction Studies"],"prefix":"10.1002","volume":"358","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8213-3813","authenticated-orcid":false,"given":"Shalu","family":"Thakur","sequence":"first","affiliation":[{"name":"Department of Chemistry &amp; Centre of Advanced Studies in Chemistry Panjab University Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohit","family":"Sharma","sequence":"additional","affiliation":[{"name":"Centre for Stem Cell and Tissue Engineering Panjab University Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nishima","family":"Wangoo","sequence":"additional","affiliation":[{"name":"Department of Applied Sciences, University Institute of Engineering &amp; Technology (U.I.E.T) Panjab University Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aman","family":"Bhalla","sequence":"additional","affiliation":[{"name":"Department of Chemistry &amp; Centre of Advanced Studies in Chemistry Panjab University Chandigarh India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(97)00044-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1351\/PAC-CON-09-09-39"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1773-2247(09)50086-X"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2020.12.037"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.114.093245"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2022.114255"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2012.01.085"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/17460441.2023.2284201"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsmedchemLett.9b00163"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2023.115433"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/ijd.14761"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja711125p"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112829"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00044-023-03152-5"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2020.112510"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2018.09.009"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3NJ04485i"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3OB01584J"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.3c00064"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cmdc.202300505"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2024.108112"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2014.914172"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.2174\/1570178620666230417084129"},{"key":"e_1_2_7_25_1","first-page":"10","article-title":"Chemo\u2010 Enzymatic Synthesis of Chiral 3\u2010hydroxy\u2010 Azetidin\u20102\u2010Ones","volume":"38","author":"Bari S. 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