{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T19:17:28Z","timestamp":1772997448493,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,21]],"date-time":"2025-12-21T00:00:00Z","timestamp":1766275200000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007847","name":"Natural Science Foundation of Jilin Province","doi-asserted-by":"publisher","award":["YDZJ202401457ZYTS"],"award-info":[{"award-number":["YDZJ202401457ZYTS"]}],"id":[{"id":"10.13039\/100007847","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Acetylcholinesterase (AChE) inhibitors are crucial for the symptomatic management of Alzheimer's disease (AD), with natural products\u2014particularly botanical sources like Yellow\n                    <jats:italic>Gastrodia elata<\/jats:italic>\n                    (YGE)\u2014serving as promising reservoirs of such inhibitors. Nevertheless, comprehensive screening and mechanistic characterization of their inhibitory potential remain limited. This study sought to identify potent AChE inhibitors from YGE, investigate their mechanisms of action, and assess their therapeutic prospects for AD. Methodologically, an integrated approach was employed, combining ultrafiltration\u2010liquid chromatography (UF\u2010LC) for rapid inhibitor screening, molecular docking and dynamics simulations for mechanistic insight, two\u2010stage high\u2010speed countercurrent chromatography for compound isolation, enzyme kinetics to delineate inhibition modalities, and network pharmacology to uncover relevant AD\u2010related targets. The findings identified seven active constituents with notable AChE inhibition, among which parishins A and G were obtained at high purity (98.26% and 97.26%, respectively) and exhibited mixed\u2010type inhibition with low IC\n                    <jats:sub>50<\/jats:sub>\n                    values (0.0145 and 0.0148\u2009mM). Molecular dynamics and network pharmacology analyses further revealed critical interactions between these compounds and key AD\u2010related targets, including ACHE, BCHE, BACE1, and PTGS2. In summary, this work underscores the potential of YGE\u2010sourced compounds, especially parishins A and G, as effective AChE inhibitors. The established integrative computational platform facilitates multi\u2010dimensional bioactivity evaluation and enables hierarchical prioritization of candidate compounds, thereby offering a valuable framework for advancing natural product\u2010derived therapeutics for AD.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70174","type":"journal-article","created":{"date-parts":[[2025,12,22]],"date-time":"2025-12-22T06:42:32Z","timestamp":1766385752000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi\u2010Angle Bioactivity Cartography for Computational Screening and Mechanistic Analysis of AChE Inhibitors From Yellow\n                    <i>Gastrodia elata<\/i>"],"prefix":"10.1002","volume":"358","author":[{"given":"Ruijun","family":"Sun","sequence":"first","affiliation":[{"name":"Central Laboratory Changchun Normal University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuchi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Central Laboratory Changchun Normal University Changchun China"},{"name":"Institute of Innovation Science and Technology Changchun Normal University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingying","family":"Xu","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy the Affiliated Hospital to Changchun University of Chinese Medicine Chagnchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Chen","sequence":"additional","affiliation":[{"name":"Central Laboratory Changchun Normal University Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunming","family":"Liu","sequence":"additional","affiliation":[{"name":"Central Laboratory Changchun Normal University Changchun 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USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sainan","family":"Li","sequence":"additional","affiliation":[{"name":"Central Laboratory Changchun Normal University Changchun 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