{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T00:12:05Z","timestamp":1778803925263,"version":"3.51.4"},"reference-count":80,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T00:00:00Z","timestamp":1771804800000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sideroxylonal B, a natural dimeric acylphloroglucinol, demonstrates promising anticancer potential through a dual mechanism of action involving both cytotoxicity and selective enzyme inhibition. In preliminary screening against the NCI 60 human tumor cell line panel at a concentration of 10\n                    <jats:sup>\u20135<\/jats:sup>\n                    M, sideroxylonal B exhibited moderate but selective antiproliferative activity, particularly against leukemia (CCRF\u2010CEM, RPMI\u20108226), breast (MCF7), and renal (CAKI\u20101) cancer cell lines. Notably, sideroxylonal B selectively inhibits tumor\u2010associated carbonic anhydrase isoforms hCA IX and XII (\n                    <jats:italic>\n                      K\n                      <jats:sub>i<\/jats:sub>\n                    <\/jats:italic>\n                    \u2009=\u200928.1 and 44.4\u2009\u00b5M), with minimal inhibition of isoforms hCA I and II. Molecular docking and MM\u2010GBSA binding energy simulations corroborated its selective interaction profile, highlighting its preferential binding to the more accommodating active sites of hCA IX and XII\n                    <jats:italic>via<\/jats:italic>\n                    hydrogen bonds and \u03c0\u2013\u03c0 interactions. The compound showed no antiviral activity against coxsackievirus B3 (CVB3) or Human Herpes virus type 1 (HHV\u20101). These findings suggest sideroxylonal B as a novel and selective natural carbonic anhydrase inhibitor with a unique structural scaffold, to be further explored for its potential application in targeted cancer therapy.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70216","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T10:15:49Z","timestamp":1772964949000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sideroxylonal B: A Dimeric Phloroglucinol From\n                    <i>Eucalyptus cinerea<\/i>\n                    With In Vitro Carbonic Anhydrase Inhibition and Evaluation of Cytotoxicity and Antiviral Potential"],"prefix":"10.1002","volume":"359","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5968-1141","authenticated-orcid":false,"given":"Fatema R.","family":"Saber","sequence":"first","affiliation":[{"name":"Pharmacognosy Department, Faculty of Pharmacy Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5520-740X","authenticated-orcid":false,"given":"Alessandro","family":"Bonardi","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Pharmaceutical and Nutraceutical Section and Laboratory of Molecular Modeling Cheminformatics &amp; QSAR University of Florence  Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6785-6394","authenticated-orcid":false,"given":"Rasha A.","family":"Hassan","sequence":"additional","affiliation":[{"name":"Pharmaceutical Organic Chemistry Department, Faculty of Pharmacy Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shymaa I. A.","family":"Abdel\u2010dayem","sequence":"additional","affiliation":[{"name":"Pharmacognosy Department, Faculty of Pharmacy Cairo University  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0669-7481","authenticated-orcid":false,"given":"Simone","family":"Giovannuzzi","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Pharmaceutical and Nutraceutical Section and Laboratory of Molecular Modeling Cheminformatics &amp; QSAR University of Florence  Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1626-680X","authenticated-orcid":false,"given":"\u0141ukasz","family":"\u015awi\u0105tek","sequence":"additional","affiliation":[{"name":"Department of Virology with Viral Diagnostics Laboratory Medical University of Lublin  Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7527-1655","authenticated-orcid":false,"given":"Reem K.","family":"Arafa","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Program University of Science and Technology, Zewail City of Science and Technology  Cairo Egypt"},{"name":"Drug Design and Discovery Lab Zewail City of Science and Technology  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ragaa","family":"Yasser","sequence":"additional","affiliation":[{"name":"Biomedical Sciences Program University of Science and Technology, Zewail City of Science and Technology  Cairo Egypt"},{"name":"Drug Design and Discovery Lab Zewail City of Science and Technology  Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9137-2509","authenticated-orcid":false,"given":"Paola","family":"Gratteri","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Pharmaceutical and Nutraceutical Section and Laboratory of Molecular Modeling Cheminformatics &amp; QSAR University of Florence  Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8164-5753","authenticated-orcid":false,"given":"Benita","family":"Hry\u0107","sequence":"additional","affiliation":[{"name":"Department of Natural Products Chemistry Medical University of Lublin  Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9313-5929","authenticated-orcid":false,"given":"Krystyna","family":"Skalicka\u2010Wo\u017aniak","sequence":"additional","affiliation":[{"name":"Department of Natural Products Chemistry Medical University of Lublin  Lublin Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-0323","authenticated-orcid":false,"given":"Claudiu T.","family":"Supuran","sequence":"additional","affiliation":[{"name":"NEUROFARBA Department, Pharmaceutical and Nutraceutical Section and Laboratory of Molecular Modeling Cheminformatics &amp; 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