{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T14:33:34Z","timestamp":1774622014522,"version":"3.50.1"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Anticoagulant\u2010dependent pseudothrombocytopenia (PTCP) is an in vitro artifact that leads to falsely low platelet counts. To evaluate the efficacy of Amikacin in reversing platelet (PLT) clumping induced by various anticoagulants, including EDTA, heparin, and sodium citrate. we collected 26 blood samples demonstrating EDTA\u2010dependent pseudothrombocytopenia. Amikacin solution (50\u2009\u03bcL of 50\u2009mg\/L) was added to 500\u2009\u03bcL of whole blood from each sample. Complete blood count (CBC) analysis was performed at 5, 30, 60, 240\u2009min, and 24\u2009h post\u2010treatment. Additionally, five samples exhibiting PLT aggregation dependent on multiple anticoagulants (EDTA, heparin, and sodium citrate) were treated with Amikacin, and PLT counts were compared across the different anticoagulant tubes. For the Amikacin\u2010treated EDTA tubes, differential leukocyte counts (DLC) were analyzed and compared with untreated controls, with validation by flow cytometry. Amikacin treatment significantly increased PLT counts in EDTA\u2010dependent samples compared with untreated controls (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.001), with the maximum effect observed at 240\u2009min. In samples with multi\u2010anticoagulant dependency, PLT counts in heparin\u2010 and sodium citrate\u2010anticoagulated tubes remained significantly lower than those in EDTA tubes after Amikacin treatment (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). Furthermore, in Amikacin\u2010treated EDTA blood, the proportions of neutrophils and basophils were significantly increased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001), whereas the proportions of lymphocytes and monocytes decreased (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.01). Amikacin effectively reverses EDTA\u2010dependent PLT clumping within 240\u2009min, but only partially resolves aggregation induced by heparin or sodium citrate. Moreover, the use of Amikacin in EDTA\u2010anticoagulated blood compromises the accuracy of CBC differential counts, rendering such samples unsuitable for routine hematological analysis.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70230","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T16:21:35Z","timestamp":1774282895000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Amikacin Mediated Platelet Aggregate Dissociation in Multi\u2010Anticoagulant\u2010Dependent Pseudothrombocytopenia (PTCP)"],"prefix":"10.1002","volume":"359","author":[{"given":"Bo","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Shanghai Ninth People's Hospital Shanghai JiaoTong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junwen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratorial The Children's Hospital of Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenying","family":"Yao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Shanghai Ninth People's Hospital Shanghai JiaoTong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shulin","family":"Ruan","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Shanghai Ninth People's Hospital Shanghai JiaoTong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunxia","family":"Xu","sequence":"additional","affiliation":[{"name":"Disinfection Supply Room, Shanghai Ninth People's Hospital Shanghai JiaoTong University School of Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhengrong","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Putuo Hospital Shanghai University of Traditional Chinese Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.22.4.460"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2011.013"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2011-0892"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2257.2006.00870.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/09537104.2012.731545"},{"issue":"6","key":"e_1_2_7_7_1","first-page":"1235","article-title":"Effects of Talaromyces Marneffei on Complete Blood Count by a Sysmex 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