{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:07:19Z","timestamp":1790690839794,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006407","name":"Natural Science Foundation of Henan Province","doi-asserted-by":"publisher","award":["252300423139"],"award-info":[{"award-number":["252300423139"]}],"id":[{"id":"10.13039\/501100006407","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>S\u2010guanylation is an endogenous posttranslational modification that has been implicated as a specific autophagic signal, yet its interacting proteins and underlying molecular mechanism remain largely unknown. Here, we developed the first diazirine\u2010based S\u2010guanylation photoaffinity probe (SGPP) to capture and identify its interacting proteins in living cells. The SGPP efficiently labeled target proteins in a time\u2010, concentration\u2010, and UV\u2010crosslinking\u2010dependent manner, as demonstrated by in\u2010gel fluorescence scanning. Using a chemoproteomic workflow combining SGPP\u2010mediated photocrosslinking, affinity enrichment, and mass spectrometry, we identified nine candidate binding proteins in HeLa cells, including ACAP2, APOD, DLGAP5, FAM83A, GNB4, LCN1, LSM4, OR5AC2, and RNASEH2A. Orthogonal anti\u2011GFP pull\u2011down validation confirmed GNB4 as a bona fide binding partner. Notably, the association of several candidates with guanine nucleotide binding activity validates the reliability of our chemoproteomic identification. To validate the degradation activity of the S\u2010guanylation warhead and to establish a functionally relevant orthogonal model for validating candidate binding proteins, we established a tetracycline (TetON) inducible expression system, which overcame the limitations of strong constitutive promoters and demonstrated efficient S\u2010guanylation\u2010induced degradation of the target protein. Collectively, this study provides the first chemical tool for studying S\u2010guanylation and identifies GNB4 as a validated binding partner, establishing a foundation for future screening of small molecule drugs that target selective autophagy.<\/jats:p>","DOI":"10.1002\/ardp.70345","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T07:14:03Z","timestamp":1790061243000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Photoaffinity Probe for S\u2011Guanylation Posttranslational Modification to Identify Its Interacting Proteins"],"prefix":"10.1002","volume":"359","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8578-6780","authenticated-orcid":false,"given":"Han","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou China"},{"name":"Henan Key Laboratory of Precision Clinical Pharmacy, The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyan","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou China"},{"name":"Henan Key Laboratory of Precision Clinical Pharmacy, The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanghe","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou China"},{"name":"Henan Key Laboratory of Precision Clinical Pharmacy, The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyu","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The First Affiliated Hospital of Zhengzhou University Zhengzhou China"},{"name":"Henan Key Laboratory of Precision Clinical Pharmacy, The First Affiliated Hospital of Zhengzhou University Zhengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1141448"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.7b00077"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4MD00787E"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41589-025-02056-2"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-025-00870-z"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2007.33"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2013.10.024"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2019.09.009"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jmedchem.3c00861"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/164_2016_5000"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2021.05.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43586-024-00308-4"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.12.5547"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.130192197"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.08.002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.181974"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.20.5789"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.07.011"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444909031011"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2023.108385"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-2181"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2024.116372"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky869"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1081"}],"container-title":["Archiv 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