{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:58:31Z","timestamp":1791381511055,"version":"4.3.3"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A series of phenazine derivatives with C\u20103 or C\u20104 benzylamino substituents were synthesized via Buchwald\u2013Hartwig amination and evaluated for antichlamydial activity against\n                    <jats:italic>Chlamydia trachomatis<\/jats:italic>\n                    serovars L2 and D. All compounds exhibited micromolar inhibitory activity, with compound\n                    <jats:bold>7d<\/jats:bold>\n                    showing the most potent effect (IC\n                    <jats:sub>50<\/jats:sub>\n                    \u2009&lt;\u20091\u2009\u03bcM) but significant host cell cytotoxicity. In contrast, compounds\n                    <jats:bold>5g<\/jats:bold>\n                    and\n                    <jats:bold>7c<\/jats:bold>\n                    demonstrated favorable selectivity, with potent antichlamydial activity and no cytotoxicity at 16\u2009\u03bcM. Mechanistic studies revealed that\n                    <jats:bold>5g<\/jats:bold>\n                    and\n                    <jats:bold>7c<\/jats:bold>\n                    inhibit\n                    <jats:italic>Chlamydia<\/jats:italic>\n                    through a dual mechanism: they block the initial stage of infection by targeting host cells, and they also interfere with later stages of intracellular reticulate body (RB) development. This represents the first report of phenazine derivatives acting via host cell targeting rather than direct action on elementary bodies (EBs). Structure\u2013activity relationship analyses indicated that the electronic nature and chain length of substituents significantly influence both activity and cytotoxicity. Collectively, these findings identify\n                    <jats:bold>5g<\/jats:bold>\n                    and\n                    <jats:bold>7c<\/jats:bold>\n                    as promising lead compounds for the development of novel antichlamydial agents with a unique mechanism of action.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70351","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:31:43Z","timestamp":1791379903000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synthesis and Antichlamydial Activity of Novel Benzylaminophenazines Against\n                    <i>Chlamydia trachomatis<\/i>"],"prefix":"10.1002","volume":"359","author":[{"given":"Yunfeng","family":"Ge","sequence":"first","affiliation":[{"name":"School of Pharmacy Nantong University Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nantong University Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Sang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nantong University Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyang","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nantong University Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengming","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nantong University Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0115-9489","authenticated-orcid":false,"given":"Yu","family":"Zhao","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nantong University Nantong China"},{"name":"Jiangsu Key Laboratory of Inflammation and Molecular Targets Nantong University Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9210-5322","authenticated-orcid":false,"given":"Xiaofeng","family":"Bao","sequence":"additional","affiliation":[{"name":"School of Pharmacy Nantong University Nantong China"},{"name":"Jiangsu Key Laboratory of Inflammation and Molecular Targets Nantong University Nantong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","first-page":"E844","DOI":"10.1503\/cmaj.221849","article-title":"A Practical Approach to the Diagnosis and Management of Chlamydia and Gonorrhea","volume":"195","author":"Van Ommen C. 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