{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:52:27Z","timestamp":1791561147989,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T00:00:00Z","timestamp":1791504000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Archiv der Pharmazie"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>Type 2 diabetes mellitus is characterized by a progressive decline in insulin sensitivity and represents an important therapeutic target for improving glucose homeostasis. Chromen\u2010derived compounds have attracted interest as potential antidiabetic agents because of their diverse pharmacological activities. Drug\u2010likeness and pharmacokinetic properties were evaluated using ADME profiling. In silico docking and simulation analyses were performed to characterize ligand\u2013protein interactions and evaluate the stability complex with carbohydrate\u2010hydrolyzing enzymes. In vitro studies included MTT assay for cell viability\/tolerability, \u03b1\u2010glucosidase and \u03b1\u2010amylase inhibition assays and 2\u2010deoxyglucose (2\u2010DG) uptake in 3T3\u2010L1 cells. Conventional RT\u2010PCR was further performed to assess PI3K and GLUT\u20104 mRNA expression. The 2\u2010DG assay provided a functional measure of cellular glucose uptake under the experimental conditions and was not designed to directly establish insulin sensitization. Both compounds showed favorable predicted drug\u2010like and pharmacokinetic properties. Docking and MD analyses predicted favorable ligand\u2013enzyme interactions and complex stability. Experimentally, 7\u2010methoxy\u20103\u2010Phenyl\u20104H\u2010Chromen\u20104\u2010one showed stronger \u03b1\u2010glucosidase inhibition, higher 2\u2010DG uptake and marked upregulation of PI3K and GLUT\u20104 expression, whereas 7,8\u2010Dihydroxy\u20103\u2010(4\u2010hydroxyphenyl)\u20104H\u2010chromen downregulated PI3K, modestly increased GLUT\u20104 and showed lower 2\u2010DG uptake. The findings indicate potential antidiabetic activity through complementary effects on carbohydrate digestion and cellular glucose handling. 7\u2010methoxy\u20103\u2010Phenyl\u20104H\u2010Chromen\u20104\u2010one showed the most consistent response among the chromen derivatives evaluated and emerged as a candidate for further investigation. The present findings do not establish direct activation of insulin\u2010signaling pathway or reversal of insulin resistance. Comprehensive mechanistic investigations, together with in vivo validation, are needed to further substantiate the therapeutic potential of the compound.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ardp.70361","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:16:15Z","timestamp":1791558975000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated In Silico and In Vitro Investigation of Chromene Derivatives as Potential Antidiabetic Agents in 3T3\u2010L1 Cells"],"prefix":"10.1002","volume":"359","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8457-0489","authenticated-orcid":false,"given":"Ankit","family":"Verma","sequence":"first","affiliation":[{"name":"Department of Pharmacology School of Pharmaceutical Education and Research, Jamia Hamdard New Delhi Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Animesh","family":"Majumdar","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Medicinal Chemistry and Molecular Modelling Lab, School of Pharmaceutical Education and Research, Jamia Hamdard New Delhi Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0322-2509","authenticated-orcid":false,"given":"Ozair","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Chemistry Medicinal Chemistry and Molecular Modelling Lab, School of Pharmaceutical Education and Research, Jamia Hamdard New Delhi Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4013-6626","authenticated-orcid":false,"given":"Manju","family":"Sharma","sequence":"additional","affiliation":[{"name":"Department of Pharmacology School of Pharmaceutical Education and Research, Jamia Hamdard New Delhi Delhi India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,9]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-7843.2008.00223.x"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/hsr2.2004"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.2991\/jegh.k.191028.001"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.283"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/1758-5996-2-49"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40842-025-00247-3"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/medsci13030087"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.116.022194"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12933-025-02966-4"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.201700223"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2022.133012"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2020.00623"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.2174\/1389557522666220331161636"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2014.03.047"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1017\/jns.2016.41"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4RA02143F"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/jfb14030126"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2025.108338"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.4314\/tjpr.v20i1.15"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13197-014-1676-0"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.4103\/2394-6555.180156"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.4c00771"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btaf259"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms222312827"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.1987"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/9889113"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.27173"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.4331\/wjbc.v11.i3.76"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2025.13773"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.3791\/55743"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21010234"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/5561129"},{"key":"e_1_2_12_34_1","first-page":"179","volume-title":"Tetrazolium (MTT) Assay for Cellular Viability and Activity","author":"Morgan D. M. L.","year":"1998"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-080-5_20"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.4103\/0253-7613.161270"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2199-10-11"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng073"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-012-0188-9"}],"container-title":["Archiv der Pharmazie"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ardp.70361","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ardp.70361","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ardp.70361","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:16:17Z","timestamp":1791558977000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ardp.70361"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":38,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/ardp.70361"],"URL":"https:\/\/doi.org\/10.1002\/ardp.70361","archive":["Portico"],"relation":{},"ISSN":["0365-6233","1521-4184"],"issn-type":[{"value":"0365-6233","type":"print"},{"value":"1521-4184","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-08-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70361"}}