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It is not well\u2010defined which ion channels are responsible for membrane potential dynamics in neutrophils, although ion channel signaling has been linked to neutrophil behavior. Here, we investigated the contribution of K\n                    <jats:sup>+<\/jats:sup>\n                    channels that are important players in different immune cells: K\n                    <jats:sub>Ca<\/jats:sub>\n                    3.1, K\n                    <jats:sub>ir<\/jats:sub>\n                    2.1, and K\n                    <jats:sub>V<\/jats:sub>\n                    1.3. We studied their roles in membrane potential regulation, chemotaxis, and LTB4 secretion using live\u2010cell imaging, analytical methods, and mathematical modeling. We identify membrane potential dynamics as an important regulatory axis in neutrophil migration. K\n                    <jats:sub>ir<\/jats:sub>\n                    2.1 is a key regulator of resting membrane potential. K\n                    <jats:sub>ir<\/jats:sub>\n                    2.1 blockade depolarizes the membrane potential of neutrophils by ~30\u2009mV to \u221241.9\u2009mV, and it delays and attenuates the C5a\u2010induced depolarization. In contrast, K\n                    <jats:sub>Ca<\/jats:sub>\n                    3.1 and K\n                    <jats:sub>V<\/jats:sub>\n                    1.3 inhibition neither modifies the resting membrane potential nor the C5a\u2010induced depolarization. Nonetheless, the inhibition of all three K\n                    <jats:sup>+<\/jats:sup>\n                    channels impairs chemotaxis toward C5a, in part by a reduced LTB\n                    <jats:sub>4<\/jats:sub>\n                    secretion and\/or possibly by non\u2010conductive properties related to the interactome of the three K\n                    <jats:sup>+<\/jats:sup>\n                    channels. Together, these findings demonstrate that K\n                    <jats:sub>Ca<\/jats:sub>\n                    3.1, K\n                    <jats:sub>V<\/jats:sub>\n                    1.3 and K\n                    <jats:sub>ir<\/jats:sub>\n                    2.1 channels play distinct but critical roles in neutrophil function and inflammatory signalling.\n                  <\/jats:p>","DOI":"10.1002\/ardp.70365","type":"journal-article","created":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T08:26:06Z","timestamp":1792139166000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["K\n                    <sup>+<\/sup>\n                    Channels in Membrane Potential Dynamics and Migration of Neutrophil Granulocytes"],"prefix":"10.1002","volume":"359","author":[{"given":"Tahir","family":"Ulu","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Physiologie II, University M\u00fcnster M\u00fcnster 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Borrego","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Cell Biology Faculty of Medicine, University of Debrecen Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6227-3301","authenticated-orcid":false,"given":"Gy\u00f6rgy","family":"Panyi","sequence":"additional","affiliation":[{"name":"Department of Biophysics and Cell Biology Faculty of Medicine, University of Debrecen Debrecen Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9280-2099","authenticated-orcid":false,"given":"Albrecht","family":"Schwab","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Physiologie II, University M\u00fcnster M\u00fcnster 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