{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T23:14:03Z","timestamp":1784848443727,"version":"3.55.0"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":69,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"},{"start":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T00:00:00Z","timestamp":1773187200000},"content-version":"vor","delay-in-days":69,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["arthroscopyjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Arthroscopy"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>To\u00a0evaluate the anti\u2010inflammatory and disease\u2010modifying potential of bone marrow aspirate concentrate (BMAC) and amniotic suspension allograft (ASA) in a chondrocyte and synovium coculture model of osteoarthritis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Seventeen patients were enrolled for cartilage, synovium, and bone marrow aspirate tissue donation prior to a total knee arthroplasty. Cartilage and synovium explants were cocultured into four different groups: one baseline group, one control group (96\u2010hour coculture), BMAC group, and ASA group. Interleukin\u20101\u03b2 (IL\u20101\u03b2), interleukin\u20106 (IL\u20106), and tumor necrosis factor\u2010alpha (TNF\u2010\u03b1) were measured at 96\u2009hours in the media with enzyme\u2010linked immunosorbent assay. Collagen type 1 \u03b1 1, Collagen type 2 \u03b1 2, Collagen type 3 \u03b1 1, aggrecan, and Cartilage Oligomeric Matrix Protein were measured in the cartilage and synovium by reverse transcription polymerase chain reaction. Safranin\u2010O staining were performed on all groups and scored by the modified Mankin scoring system.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Samples treated with ASA showed a significantly lower concentration of IL\u20101\u03b2 when compared to control samples (11.2\u2009\u00b1\u200913.9 vs 20.2\u2009\u00b1\u200939.5 pg\/mL,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.04). Treatment with BMAC was associated with a significantly lower concentrations of IL\u20106 when compared to control samples (607.32\u2009\u00b1\u2009271.07 vs 767.11\u2009\u00b1\u200930.84,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u2009.02). No significant differences were observed in gene expression within chondrocytes and synoviocytes or for Mankin scoring between treatment and control groups.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Osteoarthritic chondrocytes and synovial tissue may respond to BMAC and ASA by a reduction in IL\u20101\u03b2 and IL\u20106 concentrations, but short\u2010term cytokine responses are variable. The mechanism of response remains unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance<\/jats:title>\n                    <jats:p>This study aims to reveal the mechanism of BMAC and ASA by which these biologics function in a model of an arthritic joint.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/arj.70021","type":"journal-article","created":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T14:09:06Z","timestamp":1773238146000},"page":"263-272","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Amniotic Suspension Allograft and Bone Marrow Aspirate Concentrate Results in Highly Variable Proinflammatory Cytokines in a Coculture Model of Osteoarthritis"],"prefix":"10.1002","volume":"42","author":[{"given":"Charles P.","family":"Hannon","sequence":"first","affiliation":[{"name":"Rush University Medical Center  Chicago Illinois U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Navya","family":"Dandu","sequence":"additional","affiliation":[{"name":"Rush University Medical Center  Chicago Illinois U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hailey P.","family":"Huddleston","sequence":"additional","affiliation":[{"name":"Rush University Medical Center  Chicago Illinois U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8298-0206","authenticated-orcid":false,"given":"Divesh","family":"Sachdev","sequence":"additional","affiliation":[{"name":"Rush University Medical Center  Chicago Illinois U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neal B.","family":"Naveen","sequence":"additional","affiliation":[{"name":"Rush University Medical Center  Chicago Illinois U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Frisbie","sequence":"additional","affiliation":[{"name":"Colorado State University  Fort Collins Colorado U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne","family":"McIlwraith","sequence":"additional","affiliation":[{"name":"Colorado State University  Fort Collins Colorado U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2571-4128","authenticated-orcid":false,"given":"Arnavaz","family":"Hakimiyan","sequence":"additional","affiliation":[{"name":"Rush University Medical Center  Chicago Illinois U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian J.","family":"Cole","sequence":"additional","affiliation":[{"name":"Rush University Medical Center  Chicago Illinois U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3861-6049","authenticated-orcid":false,"given":"Susanna","family":"Chubinskaya","sequence":"additional","affiliation":[{"name":"Rush University Medical Center  Chicago Illinois U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9601-4217","authenticated-orcid":false,"given":"Adam B.","family":"Yanke","sequence":"additional","affiliation":[{"name":"Rush University Medical Center  Chicago Illinois 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