{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:13:27Z","timestamp":1790831607482,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,3,7]],"date-time":"2002-03-07T00:00:00Z","timestamp":1015459200000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis & Rheumatism"],"published-print":{"date-parts":[[2002,3]]},"abstract":"Abstract<\/jats:title>Objective<\/jats:title>To characterize the 15\u2010kd human SmD\u2010like autoantigen and its associated proteins previously shown to be recognized by IgM antibodies in patients with Epstein\u2010Barr virus (EBV)\u2010induced infectious mononucleosis.<\/jats:p><\/jats:sec>Methods<\/jats:title>The full\u2010length complementary DNA for the 15\u2010kd protein was expressed as recombinant protein and analyzed for reactivity using biochemical analysis and immunoprecipitation (IP).<\/jats:p><\/jats:sec>Results<\/jats:title>The 15\u2010kd protein was determined to be the human like\u2010Sm protein LSm4 (hLSm4). Rabbit antibody raised against the C\u2010terminal polypeptide immunoprecipitated a 68\u2010kd complex composed of LSm4 together with a group of smaller proteins ranging in size from 6.5 to 14 kd, consistent with the reported heptameric LSm complexes involved in U4\/U6 duplex formation and messenger RNA (mRNA) decapping\/degradation. About 80% of all anti\u2010Sm sera from patients with systemic lupus erythematosus (SLE) recognized the hLSm4 in vitro translated product, while 6.7% (29 of 434) immunoprecipitated from cell extracts hLSm4 together with the other members of the hLSm complex. Four sera (0.92%) showed apparently exclusive reactivity to the hLSm complex in the absence of reactivity to Sm core proteins in the IP assay.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>These findings document that while IgM, but not IgG, autoantibodies to LSm4 were found in sera from patients with EBV infection, IgG autoantibodies to hLSm4 are detected in a large number of anti\u2010Sm\u2010positive sera from patients with SLE. Importantly, in a small number of anti\u2010Sm sera the LSm complex can be recognized independently of the Sm core protein antigens. Our data introduce the concept that \u201cSm\u201d autoantigens include Sm as well as LSm complexes involved in the maturation and degradation of mRNA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.10220","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T12:00:41Z","timestamp":1033905641000},"page":"726-734","source":"Crossref","is-referenced-by-count":21,"title":["Autoantibody to hLSm4 and the heptameric LSm complex in anti\u2010Sm sera"],"prefix":"10.1002","volume":"46","author":[{"given":"Theophany","family":"Eystathioy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol L.","family":"Peebles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Hamel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Vaughn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward K. L.","family":"Chan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,3,7]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780251101"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.11.5495"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830151006"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"3592","DOI":"10.4049\/jimmunol.150.8.3592","article-title":"Autoantigenic epitopes of the B and D polypeptides of the U1 snRNP: analysis of domains recognized by the Y12 monoclonal anti\u2010Sm antibody and by patient sera","volume":"150","author":"Hirakata M","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/clim.1999.4745"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.12.3451"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.20.5789"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/35006676"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117781"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780250814"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"3744","DOI":"10.4049\/jimmunol.136.10.3744","article-title":"Epitopes, structural domains, and asymmetry of amino acid residues in SS\u2010B\/La nuclear protein","volume":"136","author":"Chan EKL","year":"1986","journal-title":"J Immunol"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.6.1627"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.25.12317"},{"key":"e_1_2_6_17_2","first-page":"928","volume-title":"Manual of clinical laboratory immunology","author":"Chan EKL","year":"1997"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.17.1.163"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(00)00098-3"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80550-4"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400803"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1994.1014"},{"key":"e_1_2_6_23_2","first-page":"289","article-title":"Potential role of the Epstein\u2010Barr virus in systemic lupus erythematosus autoimmunity","volume":"16","author":"Incaprera M","year":"1998","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.147.2.582"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.6.2341"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(70)90057-4"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6164096"},{"key":"e_1_2_6_28_2","doi-asserted-by":"crossref","first-page":"5017","DOI":"10.4049\/jimmunol.158.10.5017","article-title":"Human anti\u2010nuclear ribonucleoprotein antigen autoimmune sera contain a novel subset of autoantibodies that stabilizes the molecular interaction of U1RNP\u2010C protein with the Sm core proteins","volume":"158","author":"Satoh M","year":"1997","journal-title":"J Immunol"}],"container-title":["Arthritis & Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.10220","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.10220","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T01:15:09Z","timestamp":1700702109000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.10220"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,3]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,3]]}},"alternative-id":["10.1002\/art.10220"],"URL":"https:\/\/doi.org\/10.1002\/art.10220","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,3]]}}}