{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:25:49Z","timestamp":1791120349532,"version":"4.1.0"},"reference-count":78,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,5,8]],"date-time":"2002-05-08T00:00:00Z","timestamp":1020816000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the roles played by the neutrophil Fc\u03b3 receptor type II (Fc\u03b3RII) (CD32) and Fc\u03b3RIIIb (CD16) in phagocytosis, bacterial killing, and activation by immune complexes (ICs) and to test the hypothesis that inhibition of pathologic effector neutrophil function is possible without compromising host defense.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Receptor function was probed by enzymic removal of Fc\u03b3RIIIb from the cell surface and by use of Fab\/F(ab\u2018)<jats:sub>2<\/jats:sub> fragments of monoclonal antibodies to block receptor\u2010ligand binding. Cells were challenged with (a) serum\u2010opsonized <jats:italic>Staphylococcus aureus<\/jats:italic>, (b) serum\u2010 and IgG\u2010opsonized latex particles, and (c) synthetic soluble and insoluble ICs to mimic bacterial and inflammatory stimuli.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Phosphatidylinositol\u2010phospholipase C treatment removed &gt;97% of surface Fc\u03b3RIIIb from neutrophils previously treated with tumor necrosis factor \u03b1 to mobilize intracellular stores of receptor. This treatment profoundly inhibited activation of primed neutrophils by soluble ICs of the type found in diseased rheumatoid joints, but had no effect on phagocytosis and killing of serum\u2010opsonized <jats:italic>S aureus<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Fc\u03b3RIIIb plays a major role in the secretion of toxic products in response to ICs, but little or no role in the phagocytosis and killing of serum\u2010opsonized bacteria. The selective suppression of effector neutrophil function is therefore possible. Fc\u03b3RIIIb, or its intracellular signaling pathway, is a potential therapeutic target in inflammatory diseases such as rheumatoid arthritis, because disruption of its function should decrease inflammatory tissue damage, but not jeopardize host protection against infection.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.10230","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T12:20:06Z","timestamp":1033906806000},"page":"1351-1361","source":"Crossref","is-referenced-by-count":96,"title":["Differential role of neutrophil Fc\u03b3 receptor IIIB (CD16) in phagocytosis, bacterial killing, and responses to immune complexes"],"prefix":"10.1002","volume":"46","author":[{"given":"Gianluca","family":"Fossati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Moots","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger C.","family":"Bucknall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven W.","family":"Edwards","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,5,8]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/CBO9780511608421"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(97)01087-6"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-857X(21)00463-4"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.2001.01156.x"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(00)00481-2"},{"key":"e_1_2_5_7_2","first-page":"15","article-title":"Role of reactive oxygen and nitrogen species in etiopathogenesis of rheumatoid arthritis","volume":"18","author":"Bauerova K","year":"1999","journal-title":"Gen Physiol Biophys"},{"key":"e_1_2_5_8_2","first-page":"283","article-title":"Neutrophil functions in rheumatoid arthritis","volume":"42","author":"Nada R","year":"1999","journal-title":"Indian J Pathol Microbiol"},{"key":"e_1_2_5_9_2","first-page":"485","article-title":"Cytosolic Ca2+ signalling in inflammatory neutrophils: implications for rheumatoid arthritis","volume":"1","author":"Davies EV","year":"1998","journal-title":"Int J Mol Med"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/37.2.126"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1997.00249.x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.52.5.354"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.3.1601"},{"key":"e_1_2_5_14_2","first-page":"233","article-title":"Oxidative burst of neutrophils in patients with rheumatoid arthritis: influence of various cytokines and medication","volume":"15","author":"Mur E","year":"1997","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_5_15_2","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.4049\/jimmunol.161.3.1422","article-title":"Covalent cross\u2010linking of immune complexes by oxygen radicals and nitrite","volume":"161","author":"Uesugi M","year":"1998","journal-title":"J Immunol"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01541227"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02247841"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.55.4.248"},{"key":"e_1_2_5_19_2","first-page":"1436","article-title":"Cartilage destruction via the synovial fluid in rheumatoid arthritis","volume":"22","author":"Mohr W","year":"1995","journal-title":"J Rheumatol"},{"key":"e_1_2_5_20_2","first-page":"235","article-title":"Elastase activity in cartilage extracts and synovial fluids from subjects with osteoarthritis or rheumatoid arthritis: the prominent role of metalloproteinases","volume":"14","author":"Chevalier X","year":"1996","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.1996.0064"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1997.tb46251.x"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.188.75"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.58.9.573"},{"key":"e_1_2_5_25_2","first-page":"1510","article-title":"Concentrations and origins of soluble interleukin 6 receptor\u2010alpha in serum and synovial fluid","volume":"24","author":"Desgeorges A","year":"1997","journal-title":"J Rheumatol"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1996.0048"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.54.11.930"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780370613"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(00)89077-5"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/paa.1999.111.5.390"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.3109\/09687689809074522"},{"key":"e_1_2_5_32_2","doi-asserted-by":"crossref","first-page":"409","DOI":"10.4049\/jimmunol.159.1.409","article-title":"Fc receptor\u2010triggered insertion of secretory granules into the plasma membrane of human neutrophils: selective retrieval during phagocytosis","volume":"159","author":"Tapper H","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_5_33_2","first-page":"387","article-title":"The releasibility of lysosomal enzymes from neutrophil leukocytes in patients with rheumatoid arthritis","volume":"14","author":"Marino I","year":"1996","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_5_34_2","first-page":"59","article-title":"Effect of immune complexes in serum from patients with rheumatoid vasculitis on the expression of cell adhesion molecules on polymorphonuclear cells","volume":"19","author":"Haruta K","year":"2001","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_5_35_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1995.0019"},{"key":"e_1_2_5_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1994.tb00450.x"},{"key":"e_1_2_5_37_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2860345"},{"key":"e_1_2_5_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.1992.tb01468.x"},{"key":"e_1_2_5_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.58.4.403"},{"key":"e_1_2_5_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1997.tb46262.x"},{"key":"e_1_2_5_41_2","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1182\/blood.V75.6.1211.1211","article-title":"Neutrophil Fc\u2010\u03b3 receptors: a two\u2010way bridge in the immune system","volume":"75","author":"Huizinga TW","year":"1990","journal-title":"Blood"},{"key":"e_1_2_5_42_2","first-page":"330","article-title":"Fc\u03b3 receptors of phagocytes","volume":"126","author":"De Haas M","year":"1995","journal-title":"J Lab Clin Med"},{"key":"e_1_2_5_43_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2362.2001.00881.x"},{"key":"e_1_2_5_44_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.275"},{"key":"e_1_2_5_45_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.5.1209"},{"key":"e_1_2_5_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-2478(93)90051-3"},{"key":"e_1_2_5_47_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0244"},{"key":"e_1_2_5_48_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01548.x"},{"key":"e_1_2_5_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(99)00041-3"},{"key":"e_1_2_5_50_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.53.8.515"},{"key":"e_1_2_5_51_2","first-page":"253","article-title":"Fc\u03b3R mediated phagocytosis by human neutrophil cytoplasts","volume":"23","author":"Fleit HB","year":"1999","journal-title":"Inflammation"},{"key":"e_1_2_5_52_2","doi-asserted-by":"crossref","first-page":"3187","DOI":"10.1182\/blood.V90.8.3187","article-title":"Increased phagocyte Fc\u03b3RI expression and improved Fc\u03b3 receptor\u2010mediated phagocytosis after in vivo recombinant human interferon\u2010\u03b3 treatment of normal human subjects","volume":"90","author":"Schiff DE","year":"1997","journal-title":"Blood"},{"key":"e_1_2_5_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002770050249"},{"key":"e_1_2_5_54_2","doi-asserted-by":"crossref","first-page":"2403","DOI":"10.1182\/blood.V86.6.2403.bloodjournal8662403","article-title":"Neutrophil Fc\u03b3RIIIb deficiency, nature, and clinical consequences: a study of 21 individuals from 14 families","volume":"86","author":"De Haas M","year":"1995","journal-title":"Blood"},{"key":"e_1_2_5_55_2","doi-asserted-by":"crossref","first-page":"3999","DOI":"10.1182\/blood.V86.10.3999.bloodjournal86103999","article-title":"The polymorphonuclear neutrophil Fc\u03b3RIIIb deficiency is more frequent than hitherto assumed","volume":"86","author":"Muniz\u2010Diaz E","year":"1995","journal-title":"Blood"},{"key":"e_1_2_5_56_2","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1182\/blood.V79.8.2131.2131","article-title":"Frequency of the polymorphonuclear neutrophil Fc\u03b3RIII deficiency in the French population and its involvement in the development of neonatal alloimmune neutropenia","volume":"79","author":"Fromont P","year":"1992","journal-title":"Blood"},{"key":"e_1_2_5_57_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8524"},{"key":"e_1_2_5_58_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.29.17944"},{"key":"e_1_2_5_59_2","first-page":"37","article-title":"Gamma interferon enhances the killing of Staphylococcus aureus by human neutrophils","volume":"134","author":"Edwards SW","year":"1988","journal-title":"J Gen Microbiol"},{"key":"e_1_2_5_60_2","first-page":"3591","article-title":"Oxygen\u2010dependent killing of Staphylococcus aureus by human neutrophils","volume":"133","author":"Edwards SW","year":"1987","journal-title":"J Gen Microbiol"},{"key":"e_1_2_5_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)00247-T"},{"key":"e_1_2_5_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(93)90391-J"},{"key":"e_1_2_5_63_2","doi-asserted-by":"crossref","first-page":"1432","DOI":"10.4049\/jimmunol.144.4.1432","article-title":"Phosphatidylinositol\u2010linked Fc\u03b3RIII mediates exocytosis of neutrophil granule proteins, but does not mediate initiation of the respiratory burst","volume":"144","author":"Huizinga TW","year":"1990","journal-title":"J Immunol"},{"key":"e_1_2_5_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)36736-5"},{"key":"e_1_2_5_65_2","doi-asserted-by":"crossref","first-page":"3937","DOI":"10.4049\/jimmunol.146.11.3937","article-title":"Two mechanisms for IgG Fc\u2010receptor\u2010mediated phagocytosis by human neutrophils","volume":"146","author":"Rosales C","year":"1991","journal-title":"J Immunol"},{"key":"e_1_2_5_66_2","doi-asserted-by":"crossref","first-page":"997","DOI":"10.4049\/jimmunol.146.3.997","article-title":"Fc\u03b3RIII induces actin polymerization in human neutrophils and primes phagocytosis mediated by Fc\u03b3RII","volume":"146","author":"Salmon JE","year":"1991","journal-title":"J Immunol"},{"key":"e_1_2_5_67_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.18.10671"},{"key":"e_1_2_5_68_2","doi-asserted-by":"crossref","first-page":"3702","DOI":"10.4049\/jimmunol.149.11.3702","article-title":"Evidence for cross\u2010regulation of Fc\u03b3RIIIb (CD16) receptor\u2010mediated signaling by Fc\u03b3RII (CD32) expressed on polymorphonuclear neutrophils","volume":"149","author":"Naziruddin B","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_5_69_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.14.8071"},{"key":"e_1_2_5_70_2","doi-asserted-by":"crossref","first-page":"3940","DOI":"10.4049\/jimmunol.159.8.3940","article-title":"Cross\u2010linking of Fc\u03b3RIIa and Fc\u03b3RIIIb induces different proadhesive phenotypes on human neutrophils","volume":"159","author":"Kocher M","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_5_71_2","doi-asserted-by":"publisher","DOI":"10.1042\/bst025333s"},{"key":"e_1_2_5_72_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.58.4.415"},{"key":"e_1_2_5_73_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115496"},{"key":"e_1_2_5_74_2","doi-asserted-by":"crossref","first-page":"a25","DOI":"10.2741\/A102","article-title":"Phagocytosis and intracellular killing of serum\u2010opsonized Staphylococcus aureus by mouse fibroblasts expressing human Fc\u03b3R type IIa (CD32)","volume":"1","author":"Nibbering PH","year":"1996","journal-title":"Front Biosci"},{"key":"e_1_2_5_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.1.73-81.1995"},{"key":"e_1_2_5_76_2","first-page":"624","article-title":"Role of neutrophil Fc\u03b3RIIa (CD32) and Fc\u03b3RIIIb (CD16) polymorphic forms in phagocytosis of human IgG1\u2010 and IgG3\u2010opsonized bacteria and erythrocytes","volume":"83","author":"Bredius RG","year":"1994","journal-title":"Immunology"},{"key":"e_1_2_5_77_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80066-2"},{"key":"e_1_2_5_78_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/35.12.1244"},{"key":"e_1_2_5_79_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.14.1.397"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.10230","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.10230","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T13:59:26Z","timestamp":1697551166000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.10230"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,5]]},"references-count":78,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2002,5]]}},"alternative-id":["10.1002\/art.10230"],"URL":"https:\/\/doi.org\/10.1002\/art.10230","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,5]]}}}