{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T16:03:10Z","timestamp":1792857790270,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,6,6]],"date-time":"2002-06-06T00:00:00Z","timestamp":1023321600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Paris 7 University"},{"name":"Association Rhumatisme et Travail"},{"name":"Association pour la Recherche en Pathologie Synoviale (ARPS)"},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To quantify the inflammatory cell response in rat air pouch pseudosynovial membrane during monosodium urate monohydrate (MSU) crystal\u2013induced inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In the rat air\u2010pouch model, we used a computer\u2010assisted histomorphometric method to quantify cell distributions, based on cell linear densities, in histologic sections of membranes from pouches injected with MSU or saline. The volume, white blood cell (WBC) count, and histamine content of the pouch exudates were determined at several time points.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Injection of 10 mg of MSU crystals into the pouch produced an acute exudate. After peaking at 24 hours, the exudate volume and WBC count decreased spontaneously over the next 3 days, simulating the self\u2010limited course of acute gout. Membrane thickness followed a parallel course. Membrane polymorphonuclear cell (PMN) linear densities were closely correlated with exudate WBC counts, suggesting PMN recruitment from the subintimal synovial membrane. Both monocyte\/macrophage and mast cell linear densities increased in the subintimal layer 2 hours after crystal injection (<jats:italic>P<\/jats:italic> = 0.038 and <jats:italic>P<\/jats:italic> = 0.03, respectively, versus controls), whereas PMN linear densities showed 2 peaks, one at 4 hours and the other 24 hours. The exudate histamine content peaked 6 hours after crystal injection, when mast cell linear densities were minimal in the membranes, suggesting mast cell degranulation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>An increase in monocyte\/macrophage and mast cell densities in the membrane preceded the PMN influx in the pouch membrane and exudate, suggesting that mast cells may be involved in the early phase of MSU crystal\u2013induced inflammation, at least in this rat model.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.10326","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T12:29:56Z","timestamp":1033907396000},"page":"1643-1650","source":"Crossref","is-referenced-by-count":96,"title":["Monosodium urate monohydrate crystal\u2013induced inflammation in vivo: Quantitative histomorphometric analysis of cellular events"],"prefix":"10.1002","volume":"46","author":[{"given":"C.","family":"Schiltz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Liot\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Prudhommeaux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Meunier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Champy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Callebert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Bardin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,6,6]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"102","article-title":"Urate crystal induced inflammation in dog joints: sequence of synovial changes","volume":"1","author":"Schumacher HR","year":"1974","journal-title":"J Rheumatol"},{"key":"e_1_2_5_3_2","first-page":"54","article-title":"Experimental inflammation induced by naturally occurring microcrystalline calcium salts","volume":"3","author":"Denko CW","year":"1976","journal-title":"J Rheumatol"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1027322304880"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.62.4.444"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.48.6.476"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00271688"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780260612"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780360912"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-197805000-00004"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(73)80013-9"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820231006"},{"key":"e_1_2_5_13_2","first-page":"1162","article-title":"Components generated locally as well as serum alter the phlogistic effect of monosodium urate crystals in vivo","volume":"20","author":"Ortiz\u2010Bravo E","year":"1993","journal-title":"J Rheumatol"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780390718"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01983104"},{"key":"e_1_2_5_16_2","volume-title":"Initiation aux techniques de l'histologie animale","author":"Martoja R","year":"1967"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711340205"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.051599198"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780371207"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118147"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00917514"},{"key":"e_1_2_5_22_2","first-page":"123","article-title":"Molecular determinants of monosodium urate crystal\u2010induced murine peritonitis: a role for endogenous mast cells and a distinct requirement for endothelial\u2010derived selectins","volume":"283","author":"Getting SJ","year":"1997","journal-title":"J Pharmacol Exp Ther"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.12.7492"},{"key":"e_1_2_5_24_2","first-page":"433","article-title":"Crystal induced inflammation in canine joints. 3. 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