{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:16:06Z","timestamp":1791141366748,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2002,8,9]],"date-time":"2002-08-09T00:00:00Z","timestamp":1028851200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To examine the prevalence, clinical associations, and pathogenic role of autoantibodies to c\u2010Mpl, the thrombopoietin (TPO) receptor, in patients with systemic lupus erythematosus (SLE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sera from 69 SLE patients, 84 patients with idiopathic thrombocytopenic purpura (ITP), and 60 healthy individuals were screened for anti\u2013c\u2010Mpl antibodies by enzyme\u2010linked immunosorbent assay using recombinant c\u2010Mpl as an antigen. Clinical findings, autoantibody profiles, and serum TPO levels were compared between SLE patients with and without anti\u2013c\u2010Mpl antibodies. A pathogenic role for the anti\u2013c\u2010Mpl antibody was evaluated by examining its inhibitory effect on TPO\u2010dependent cell proliferation and megakaryocyte colony formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum anti\u2013c\u2010Mpl antibody was detected in 8 SLE patients (11.6%) and 7 ITP patients (8.3%), but in none of the healthy controls. Anti\u2013c\u2010Mpl antibody was associated with thrombocytopenia (<jats:italic>P<\/jats:italic> = 0.0002) and a decrease in bone marrow megakaryocytes (<jats:italic>P<\/jats:italic> = 0.02) in SLE patients. Serum TPO levels in thrombocytopenic SLE patients with anti\u2013c\u2010Mpl antibodies were significantly elevated compared with levels in those without the antibodies (<jats:italic>P<\/jats:italic> = 0.007). IgG fractions purified from anti\u2013c\u2010Mpl antibody\u2013positive sera bound to c\u2010Mpl expressed on the cell surface and inhibited TPO\u2010dependent cell proliferation and megakaryocyte colony formation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Autoantibody to c\u2010Mpl is present in a subset of SLE patients with thrombocytopenia and megakaryocytic hypoplasia. It is likely that the impaired thrombopoiesis in these patients is mediated by the anti\u2013c\u2010Mpl antibody, which functionally blocks an interaction between TPO and c\u2010Mpl.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.10420","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T12:12:48Z","timestamp":1033906368000},"page":"2148-2159","source":"Crossref","is-referenced-by-count":71,"title":["Autoantibody to c\u2010Mpl (thrombopoietin receptor) in systemic lupus erythematosus: Relationship to thrombocytopenia with megakaryocytic 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