{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:35:04Z","timestamp":1791164104154,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2002,8,9]],"date-time":"2002-08-09T00:00:00Z","timestamp":1028851200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Deutsche Forschungsgemeinschaft-Sonderforschungsbereich","award":["421\/C7"],"award-info":[{"award-number":["421\/C7"]}]},{"name":"Deutsche Forschungsgemeinschaft-Sonderforschungsbereich","award":["Do491\/4-3"],"award-info":[{"award-number":["Do491\/4-3"]}]},{"name":"Deutsche Forschungsgemeinschaft-Sonderforschungsbereich","award":["5-1"],"award-info":[{"award-number":["5-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To delineate the mechanism of the abnormalities in B cell biology found in patients with primary Sj\u00f6gren's syndrome (SS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The distribution of peripheral B cell subpopulations in 21 patients with primary SS was analyzed by immunofluorescence labeling and flow cytometry. Immunoglobulin rearrangements were analyzed in single B cells isolated from the peripheral blood and parotid glands by fluorescence\u2010activated cell sorting.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significant reduction in the number of peripheral CD27+ memory B cells was found in SS patients, including a significantly reduced number of CD27+\/IgD+\/IgM+\/CD5+ memory B cells. Remarkably, SS patients with secondary lymphoma uniquely exhibited an increase in CD27\u2010expressing peripheral B cells, including CD27<jats:sup>high<\/jats:sup> plasmablasts. Molecular analysis for mutated Ig gene rearrangements confirmed that CD27 expression distinguished naive and memory cells in SS. In contrast to the peripheral blood, the majority of parotid B cells from 1 patient examined exhibited both the mutational status and phenotype of memory B cells. Accordingly, the mutational frequencies of V<jats:sub>H<\/jats:sub> rearrangements were significantly greater in parotid B cells than in peripheral blood B cells, whereas the V<jats:sub>H<\/jats:sub> gene repertoire appeared to be very similar between the compartments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data indicate that there is an accumulation\/retention of memory B cells in the inflamed salivary glands of SS patients. It is possible that preferential accumulation of CD27+ memory B cells in the inflamed parotid gland explains their reduction in the peripheral blood.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.10445","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T12:12:48Z","timestamp":1033906368000},"page":"2160-2171","source":"Crossref","is-referenced-by-count":237,"title":["Diminished peripheral blood memory B cells and accumulation of memory B cells in the salivary glands of patients with Sj\u00f6gren's syndrome"],"prefix":"10.1002","volume":"46","author":[{"given":"Arne","family":"Hansen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Odendahl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karin","family":"Reiter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annett M.","family":"Jacobi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugen","family":"Feist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Scholze","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd R.","family":"Burmester","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter E.","family":"Lipsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"D\u00f6rner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,8,9]]},"reference":[{"key":"e_1_2_5_2_2","series-title":"Immunological Series","first-page":"247","volume-title":"Systemic autoimmunity","author":"Harley JB","year":"1991"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-200009000-00007"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01626347"},{"key":"e_1_2_5_5_2","first-page":"39","article-title":"Vascular endothelium and lymphocyte adhesion molecules in minor salivary glands of patients with Sj\u00f6gren's syndrome","volume":"37","author":"Aziz KE","year":"1992","journal-title":"J Clin Lab Immunol"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.52.11.806"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3234"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830251010"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.1.221"},{"key":"e_1_2_5_10_2","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1093\/ajcp\/110.3.327","article-title":"Characterization of the lymphoid infiltrate in Hashimoto thyroiditis by immunohistochemistry and polymerase chain reaction for immunoglobulin heavy chain rearrangement","volume":"110","author":"Hsi ED","year":"1998","journal-title":"Am J Clin Pathol"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(97)00030-1"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.001001"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(98)80161-1"},{"key":"e_1_2_5_14_2","first-page":"577","article-title":"Expression of CD5 and CD23 on B cells of patients with rheumatoid arthritis, systemic lupus erythematosus and Sj\u00f6gren's syndrome. 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