{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:06:53Z","timestamp":1791194813775,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2002,9,27]],"date-time":"2002-09-27T00:00:00Z","timestamp":1033084800000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Cytokines and growth factors can be a target of autoantibodies in systemic inflammatory diseases. We examined whether patients with neutropenia and either Felty's syndrome (FS) or systemic lupus erythematosus (SLE) have autoantibodies against granulocyte colony\u2010stimulating factor (G\u2010CSF) and whether these autoantibodies are functionally relevant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fifteen patients with neutropenia due to FS were matched for age, sex, and disease activity with 16 normocytic rheumatoid arthritis (RA) control patients. Sixteen patients with SLE and neutropenia were matched with 16 normocytic SLE control patients. Antibodies against G\u2010CSF were measured by enzyme\u2010linked immunosorbent assay and Western blotting. Antibody specificity was verified by competitive inhibition using recombinant human G\u2010CSF. The effect of anti\u2013G\u2010CSF antibodies on the functional activity of their target molecule was measured in a bioassay using G\u2010CSF\u2013sensitive murine 32D cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IgG anti\u2013G\u2010CSF was found in 11 FS patients, 6 SLE patients with neutropenia, 6 SLE control patients, and none of the RA control patients. IgM anti\u2013G\u2010CSF was found in 6 neutropenic and 3 normocytic SLE patients. Anti\u2013G\u2010CSF antibodies were associated with an exaggerated serum level of G\u2010CSF and a low neutrophil count. A neutralizing effect of anti\u2013G\u2010CSF antibodies on its target molecule was found in 3 of the 9 patients tested. Irrespective of the presence or absence of anti\u2013G\u2010CSF antibodies, neutropenic patients with FS and SLE had exaggerated serum levels of G\u2010CSF.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Anti\u2013G\u2010CSF autoantibodies are common in neutropenia due to FS and SLE. In individual patients, these autoantibodies have a neutralizing capacity. In patients without neutralizing antibodies, hyposensitivity of the myeloid cells to G\u2010CSF appears to be central to the pathogenesis of the neutropenia in FS and SLE.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.10497","type":"journal-article","created":{"date-parts":[[2002,10,9]],"date-time":"2002-10-09T04:45:25Z","timestamp":1034138725000},"page":"2384-2391","source":"Crossref","is-referenced-by-count":91,"title":["Autoantibodies against granulocyte colony\u2010stimulating factor in Felty's syndrome and neutropenic systemic lupus erythematosus"],"prefix":"10.1002","volume":"46","author":[{"given":"Bernhard","family":"Hellmich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Csernok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Schatz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang L.","family":"Gross","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armin","family":"Schnabel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,9,27]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-199069020-00001"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0268-960X(93)90006-P"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(85)90220-7"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000441-199408000-00007"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200004)43:4<834::AID-ANR14>3.0.CO;2-H"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1995.tb03726.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780210503"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780260802"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1999.00806.x"},{"key":"e_1_2_6_11_2","first-page":"219","article-title":"Anti\u2010IL\u20101\u03b1 autoantibodies in patients with rheumatic diseases and in healthy subjects","volume":"30","author":"Suzuki H","year":"1991","journal-title":"Scand J Immunol"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"935","DOI":"10.4049\/jimmunol.152.2.935","article-title":"IL\u20106\u2010anti\u2010IL\u20106 autoantibody complexes with IL\u20106 activity in sera from some patients with systemic sclerosis","volume":"152","author":"Suzuki H","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780401216"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(199711)173:2<219::AID-JCP25>3.0.CO;2-9"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02678552"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.6.1907.bloodjournal8861907"},{"key":"e_1_2_6_17_2","first-page":"541","article-title":"Disease activity in systemic lupus erythematosus: report of the Consensus Study Group of the European Workshop for Rheumatology Research. 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