{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:50:37Z","timestamp":1791049837021,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2002,11,8]],"date-time":"2002-11-08T00:00:00Z","timestamp":1036713600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>One of the pathologic hallmarks of juvenile rheumatoid arthritis (JRA) is a tumor\u2010like expansion of inflamed synovial tissue, or pannus, which causes much of the joint damage in this disease. The expansion of pannus is supported by extensive formation of new blood vessels. We have previously shown that revascularization of minced JRA synovial tissues engrafted into SCID mice correlated with the intensity of inflammatory activity in the tissues and with interleukin\u201015 (IL\u201015) expression. Since synovial vascular endothelial cells (VECs) expressed IL\u201015 receptors, the present study was undertaken to investigate the hypothesis that IL\u201015 might play a role in neovascularization of the pannus.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To evaluate IL\u201015 for possible angiogenic activity, we assessed the ability of recombinant human IL\u201015 (rHuIL\u201015) to induce VEC growth directly and to stimulate synovial cells to produce endothelial growth factors. Since IL\u201015 had been shown to inhibit apoptosis of certain immune cells, we were also interested in whether it might have similar effects on VECs. Apoptosis was induced by addition of sodium nitroprusside (SNP) at 1\u20132 m<jats:italic>M<\/jats:italic> to &gt;80% confluent primary VECs, and numbers of apoptotic cells were determined by annexin V assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Addition of rHuIL\u201015 at 10\u2013100 ng\/ml to primary synovial fibroblast cultures failed to up\u2010regulate expression of vascular endothelial growth factor and angiopoietin 1 by these cells. Although rHuIL\u201015 failed to induce a mitogenic response of VECs, it promoted survival of these cells on Matrigel. Preincubation of VECs with rHuIL\u201015 at 50 ng\/ml significantly reduced the proportion of VECs undergoing apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>IL\u201015 promotes survival of VECs on Matrigel and inhibits SNP\u2010induced apoptosis of endothelial cells. We hypothesize that this mechanism may be relevant to the stabilization of newly formed vascular structures in JRA synovium.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.10610","type":"journal-article","created":{"date-parts":[[2002,11,12]],"date-time":"2002-11-12T18:42:32Z","timestamp":1037126552000},"page":"3010-3014","source":"Crossref","is-referenced-by-count":14,"title":["Interleukin\u201015 inhibits sodium nitroprusside\u2013induced apoptosis of synovial fibroblasts and vascular endothelial cells"],"prefix":"10.1002","volume":"46","author":[{"given":"Lin","family":"Yang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherry","family":"Thornton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexei A.","family":"Grom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,11,8]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI5929"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.175.4.1135"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35025215"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199807)41:7<1258::AID-ART17>3.0.CO;2-1"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1986"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0296-175"},{"key":"e_1_2_5_8_2","first-page":"369","article-title":"IFN gamma:IL\u20104 ratios and associated type 1 cytokine expression in JRA synovial tissue","volume":"29","author":"Scola MP","year":"2002","journal-title":"J Rheumatol"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1997.6435"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200104)44:4<794::AID-ANR135>3.0.CO;2-7"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290216"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119458"},{"key":"e_1_2_5_13_2","first-page":"1091","article-title":"Regulation of synoviocyte proliferation, apoptosis, and invasion by the p53 tumor suppressor gene","volume":"152","author":"Aupperle KR","year":"1998","journal-title":"Am J Pathol"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00844-8"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.11.6292"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200109)44:9<2024::AID-ART351>3.0.CO;2-K"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.10610","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.10610","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T12:36:29Z","timestamp":1697027789000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.10610"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11]]},"references-count":15,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2002,11]]}},"alternative-id":["10.1002\/art.10610"],"URL":"https:\/\/doi.org\/10.1002\/art.10610","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11]]}}}